The rates of specific catalytic events in the mitochondrial inner membrane were determined after enriching the membrane bilayer with exogenous phospholipid. Inner membranes were fused with soybean phospholipid, then separated into four buoyant-distinct fractions by sucrose density centrifugation. Analysis revealed a progressively increasing phospholipid to protein ratio from the least buoyant (...

Interactions of DNA and the gene product D5 (gpD5) of bacteriophage T5, a DNA-binding protein that binds preferentially and cooperatively to double-stranded DNA, were analyzed by metrizamide gradient centrifugation. Conditions were set so that DNA and DNA protein complex sedimented to apparent equilibrium positions. DNA has a buoyant density of 1.12 g/cm3, and DNA saturated with gpD5 has a buoy...

The substrate specificity of lipid transfer protein has been examined in whole plasma in vitro by following the redistribution of high density lipoprotein (HDL)-derived [3H]cholesteryl ester (CE) into apolipoprotein (apo) B-containing lipoproteins using density gradient ultracentrifugation. HDL-derived [3H]CEs were incubated with plasma or isolated lipoprotein classes (very low density lipoprot...

The aim of this paper is to present a simplified, yet rigorous, deduction the Boussinesq approximated governing equations for buoyant flows. In order carry out core procedure, simplified version manifold asymptotic analyses available in literature discussed. method adopted study focussed on local balance valid general, not necessarily Newtonian, fluid. analysis carried by demonstrating leading ...