Ferroptosis is a unique variety of non-apoptotic cell death, driven by massive lipid oxidation in an iron-dependent manner. Since ferroptosis was introduced as concept 2012, it has demonstrated its essential role the pathogenesis neurodegenerative diseases and important therapy-resistant cancer cells. Thus, detailed molecular understanding both canonical alternative pathways required. There set...

This work is the next step in studying interplay between C-2028 (anticancer-active unsymmetrical bisacridine developed our group) and glutathione S-transferase/glutathione (GST/GSH) system. Here, we analyzed concentration- pH-dependent GSH conjugation of rat liver microsomes cytosol. We also applied three recombinant human GST isoenzymes, which altered expression was found various tumors. The f...

Cellular molecules possess various mechanisms in responding to oxidant stress. In terms of protein responses, protein S-glutathionylation is a unique post-translational modification of protein reactive cysteines forming disulfides with glutathione molecules. This modification has been proposed to play roles in antioxidant, regulatory and signaling in cells under oxidant stress. Recently, the in...

Various enzymic systems, such as nitrite reductase, sulfite reductase and glutathione reductase, have been proposed for, or suspected to be involved in, the reduction of selenite in bacteria. As alphaproteobacteria have been shown to be highly tolerant to transition metal oxyanions, it seemed interesting to investigate the hypothetical involvement of these different enzymes in the reduction of ...

The biological basis for the general pharmacological resistance of human melanoma is unknown. A unique biochemical feature of the melanocyte is the synthesis of melanin, which leads to the generation of hydrogen peroxide and the consumption of reduced glutathione. This activity produces a state of chronic oxidative stress in these cells. We demonstrated previously that the expression of the c-j...

Tupling AR, Vigna C, Ford RJ, Tsuchiya SC, Graham DA, Denniss SG, Rush JW. Effects of buthionine sulfoximine treatment on diaphragm contractility and SR Ca pump function in rats. J Appl Physiol 103: 1921–1928, 2007. First published August 23, 2007; doi:10.1152/japplphysiol.00529.2007.—The purpose of this study was to examine the effects of glutathione (GSH) depletion and cellular oxidation on r...

between the charge distribution of the amide bond and the fluoroalkene moiety, as well as their dipole moments. Therefore, a monofluoroalkene moiety can be used as a peptidomimetic unit in the design of protease inhibitors; this type of rigid isostere of the peptidic bond can facilitate the cis/ trans conformational control of the replaced peptidic fragment. Numerous synthetic endeavors have be...