Diabetes mellitus is one of the most common endocrine diseases and its type 2 is the major form of diabetes, accounting for 90% of cases worldwide. One of the complications of diabetes is post-prandial hyperglycemia (PPHG). The inhibition of carbohydrate hydrolyzing enzymes such as α-amylase can be an important in the control of blood glucose levels and managing PPHG in patients with type 2 dia...

Harada, Etsumori, Megumi Mitani, and Takashi Takeuchi. Potentiation of carbachol-induced amylase release by propionate in guinea pig and vole pancreatic acini. Am. J. Physiol. 277 (Regulatory Integrative Comp. Physiol. 46): R767–R775, 1999.—The action of propionate, one of the major end products of microbial fermentation in herbivores was investigated in isolated, perifused pancreatic acini of ...

BACKGROUND Plant-based foods have been used in traditional health systems to treat diabetes mellitus. The successful prevention of the onset of diabetes consists in controlling postprandial hyperglycemia by the inhibition of α-glucosidase and pancreatic α-amylase activities, resulting in aggressive delay of carbohydrate digestion to absorbable monosaccharide. In this study, five plant-based foo...

A heat-stable raw-starch-digesting amylase (RSDA) was generated through PCR-based site-directed mutagenesis. At 65 degrees C, the half-life of this mutant RSDA, which, compared with the wild-type RSDA, lacks amino acids R178 and G179, was increased 20-fold. While the wild type was inactivated completely at pH 3.0, the mutant RSDA still retained 41% of its enzymatic activity. The enhancement of ...

Background: Diabetes is possibly the mainly common metabolic syndrome with salivary inference. However, lack of literature on possible relationship between diabetes and α-amylase, Immunoglobulin-A (IgA) flow rate. Therefore, present study aims to estimate immunoglobulin-A rate in diabetic subjects Indian population.Methods: The cross sectional was done 60 mellitus (T2DM) subjects’ non-diabetic ...

Wheat α-amylase, a multi-domain protein with immense industrial applications, belongs to α+β class of proteins with native molecular mass of 32 kDa. In the present study, the pathways leading to denaturation and the relevant unfolded states of this multi-domain, robust enzyme from wheat were discerned under the influence of temperature, pH and chemical denaturants. The structural and functional...