A genomic DNA fragment from Saccharomyces cerevisiae which contains the SRA5 (=PDE2) gene, coding for a low Km cAMP-phosphodiesterase, was transfected into Chinese hamster ovary cells. Clones carring the cAMP-phosphodiesterase gene were capable of growth in the presence of cholera toxin, which slows the growth of untransfected cells by elevating their cAMP levels. The cholera toxin-resistant tr...

A marked increase of cyclic AMP-binding and protein kinase activities occurs in the nuclei of N6,02’-dibutyryl adenosine 3’:5’-monophosphate (Bt,cAMP)-responsive Walker 256 mammary carcinoma (W256) following incubation of the tumor slices with CAMP in uitro. The macromolecular fraction containing [“HIcAMP in the nuclei can be extracted with 1.0 M KC1 and identified by acrylamide gel electrophor...

Changes in intracellular 3*,5* cyclic AMP (cAMP) concentration regulate the development of natural competence in Haemophilus influenzae. In Escherichia coli, cAMP levels are modulated by a cAMP phosphodiesterase encoded by the cpdA gene. We have used several approaches to demonstrate that the homologous icc gene of H. influenzae encodes a functional cAMP phosphodiesterase and that this gene lim...

Changes in intracellular 3',5' cyclic AMP (cAMP) concentration regulate the development of natural competence in Haemophilus influenzae. In Escherichia coli, cAMP levels are modulated by a cAMP phosphodiesterase encoded by the cpdA gene. We have used several approaches to demonstrate that the homologous icc gene of H. influenzae encodes a functional cAMP phosphodiesterase and that this gene lim...

The motile responses of Dictyostelium discoideum amoebae to a cyclic AMP (cAMP) concentration gradient were examined using a novel assay system. In this system, a cAMP concentration gradient was generated, while the overall cAMP concentration could be either increased or decreased in a chamber containing amoebae. The chemotactic responses of amoebae were examined immediately after they had been...

Second messenger cAMP regulates many cellular functions through its effectors, such as cAMP-dependent protein kinase (PKA) and Epac (exchange proteins directly activated by cAMP). Spatial and temporal control of cAMP signaling is crucial to differential regulation of cellular targets involved in various signaling cascades. To investigate the compartmentalized cAMP signaling, we constructed fluo...

The article discusses the experience of individual project activity organization with gifted children in a summer sports camp, where 64 young athletes Republic Tatarstan participated pedagogical testing and experiment were involved table tennis, wrestling, athletics showed high performance. implementation for their body self-control proposed by authors within framework “Champion's Way” educatio...

Abstract In a mouse model of Graves’ disease (GD), diosgenin has been shown to have therapeutic effect on GD by alleviating goitre. However, research the autoimmune thyroiditis (AIT) is lacking. this study, transcriptomics was used comprehensively analyse protective against AIT in rats and possible mechanism. The results showed that diosgenin-intervention group, compared expression serum triiod...

Studies of humanitarian supply chain operations contribute to efficient system designs for the delivery goods and services disaster- conflict-stricken regions. However, our understanding what happens these relief after they are delivered is quite limited. Using a case study Za'atari refugee camp in Jordan, we develop theory informal networks, whereby uncover how refugee-led, networks emerge cam...