Sleeping Beauty (SB) is the first synthetic DNA transposon that was shown to be active in a wide variety of species. Here, we review studies from the last two decades addressing both basic biology and applications of this transposon. We discuss how host-transposon interaction modulates transposition at different steps of the transposition reaction. We also discuss how the transposon was transla...

PURPOSE The objective of our study was to predicate candidate genes in cervical cancer (CC) using a network-based strategy and to understand the pathogenic process of CC. METHODS A pathogenic network of CC was extracted based on known pathogenic genes (seed genes) and differentially expressed genes (DEGs) between CC and normal controls. Subsequently, cluster analysis was performed to identify...

objective: prostate cancer is the fifth most common cancer. in 2012, it was the second leading cause of cancer death for men worldwide. the pi3k/akt pathway plays an essential role in pathogenesis of prostate cancer; the key role of this pathway in cancer progression makes it an attractive target for prostate cancer therapy. micrornas (mirnas) that regulate gene expression have a special abilit...

Real time-qPCR is the most reliable method for evaluation of mRNA expression levels. However, to obtain accurate results, selection of suitable reference genes is necessary for normalizing the real-time qPCR data. The aim of this research was to validate the expression stability of three potential reference genes (ACTB, GAPDH and UXT) in milk somatic cells of Holstein dairy cattle under differe...

resistance gene analog polymorphism (rgap) markers linked to fusarium head blight resistance (fhb) and co-localize with qfhs.ndsu-3bs were identified using f3 plants and f3:5 lines derived from a ‘wangshuibai’ (resistant) / ‘seri82’ (susceptible) cross. the mapping populations were genotyped using 50 degenerate primers designed based on the known r genes. out of the 50 designed primer combinati...

Predicting candidate genes using gene expression profiles and unbiased protein-protein interactions (PPI) contributes a lot in deciphering the pathogenesis of complex diseases. Recent studies showed that there are significant disparities in network topological features between non-disease and disease genes in protein-protein interaction settings. Integrated methods could consider their characte...

PURPOSE To identify the genetic basis of Schnyder crystalline corneal dystrophy (SCCD) through screening of positional candidate genes in affected patients. METHODS Mutation screening of fifteen genes (CORT, CLSTN1, CTNNBIP1, DFFA, ENO1, GPR157, H6PD, KIF1B, LOC440559, LZIC, MGC4399, PEX14, PGD, PIK3CD, and SSB1) that lie within the candidate gene region for SCCD was performed in members of t...

BACKGROUND Large-scale screens of the maize genome identified 48 genes that show the putative signature of artificial selection during maize domestication or improvement. These selection-candidate genes may act as quantitative trait loci (QTL) that control the phenotypic differences between maize and its progenitor, teosinte. The selection-candidate genes appear to be located closer in the geno...