glucagon like peptide-1; Ala: Alanine; Gly: Glycine; Pro: Proline; HTS: high-throughput screening; ESI: electrospray ionization; APCI: Atmospheric Pressure Chemical Ionization; pNA: p-nitroanilide; Asp: Aspartic acid; Thr: Threonine; Met: Methionine; Phe: Phenylalanine; Leu: Leucine; Ser: Serine; HOBT: Hydroxybenzotriazole ; EDCI: 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride; Me:...

Dehydrogenative desulfurization of thiourea derivatives (RNHC(S)NHR') has been achieved, to give carbodiimides (RN=C=NR'), in the reaction with hydrosilane and (η(5)-C5H5)Fe(CO)2Me. The obtained carbodiimide reacted with (η(5)-C5H5)Fe(CO)(SiR3) formed in the reaction to give an N-silylated η(2)-amidino iron complex, which was isolated and then characterized by X-ray analysis.

A practical solid-phase synthesis of deoxynucleic guanidine (DNG), a positively charged DNA backbone analogue, is reported. The nucleoside coupling step in the solid-phase synthesis of DNG involves the attack of a terminal 3′-amine upon an electronically activated 5′-carbodiimide to create a protected guanidinium internucleoside linkage. The activated carbodiimide is synthesized in situ by the ...

We have studied subunit-subunit interactions in bovine lutropin (bLH) through characterization of carbodiimide-cross-linked derivatives (EDCbLH). Spectrophotometric titrations indicated that an additional tyrosine residue ionizes abnormally in EDCbLH compared to native bLH. Furthermore, only five out of seven tyrosines in the cross-linked derivative were ionized at pH 13. Treatment with tetrani...

Cancer invasion and progression involves a motile cell phenotype, which is under complex regulation by growth factors/cytokines and extracellular matrix (ECM) components within the tumor microenvironment. Hyaluronic acid (HA) is one stromal ECM component that is known to facilitate tumor progression by enhancing invasion, growth, and angiogenesis(1). Interaction of HA with its cell surface rece...

Pig phospholipase A2, pig iso-phospholipase A2 and bovine pancreatic phospholipase A2 were reacted in solution with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, in the presence of N-hydroxysulfosuccinimide, at pH 7. The influence of micellar protectants was analyzed. In the presence of n-hexadecylphosphocholine, the losses of activity in micellar diheptanoyl-lecithin were 80, 35, and 10% in b...