Human and bovine glomerular basement membranes (GBM) were previously shown to be a three-dimensional molecular sieve composed of pores and strands by negative staining and electron microscopy. In this study, rat GBM were isolated under several different conditions to rule out morphological changes due to isolation procedures. Rat GBM isolated under different conditions all showed the same morph...

We report the quantitative, non-destructive determination of the concentration profile of an organic molecule in a nanoporous polycrystalline zeolite molecular sieve membrane, by step scan infra-red photoacoustic experiments and analysis. An important application of stepscan photoacoustic spectroscopy is the transport-model-independent experimental description of membrane transport, by simultan...

The rate of phloem loading, its selectivity, and the disposition of labeled carbon were studied following application of (14)C-labeled sugars to the free space of source leaves of sugar beet (Beta vulgaris L.). Buffered 10 mm solutions of (14)C-labeled sucrose, fructose, stachyose, mannitol, 3-0-methyl glucose or l-glucose were applied to the abraded epidermis of source leaves held in the dark....

Gonococci, grown on agar, frequently give rise to opaque colonies. This opacity phenotype is associated with the presence of one or more outer membrane proteins of approximately 28,000 mol weight. These proteins are included within a class of proteins named proteins II. A method is described to isolate and purify the opacity-associated proteins from Neisseria gonorrhoeae. This method uses high ...

How sucrose transporters (SUTs) regulate phloem unloading in monocot stems is poorly understood and particularly so for species storing high Suc concentrations. To this end, Sorghum bicolor SUTs SbSUT1 and SbSUT5 were characterized by determining their transport properties heterologously expressed in yeast or Xenopus laevis oocytes, and their in planta cellular and subcellular localization. The...