BACKGROUND This study deals with the anchorage of polyelectrolyte films onto titanium surfaces via a cathecol-based linker for biomedical applications. METHODOLOGY The following study uses a molecule functionalized with a catechol and a carboxylic acid: 3-(3,4-dihydroxyphenyl)propanoic acid. This molecule is anchored to the TiO(2) substrate via the catechol while the carboxylic acid reacts wi...

An amperometric biosensor for the determination of catechol was developed by immobilizing tyrosinase (tyr) on gold nanoparticles (AuNPs) and a (3-mercaptopropyl)-trimethoxysilane (MPTS) sol-gel three-dimensional network film-modified gold electrode. The AuNPs self-assembled in a sol-gel network provided an excellent microenvironment for an enzymatic reaction between tyrosinase and the substrate...

Infusions of 2.5-7.8 ng. noradrenaline/Kg./min. over 30 min. and intruperitonenl injections of noradrenaline and adrenaline up to 2000 Mg/Kg. in the cat had no effect on the catechol amine content of the. heart, spleen, liver, kidney and skeletal muscle, except for a slight increase in the adrenaline content. It is concluded that these organs lack the ability to accumulate i-iirulating catechol...

The aerobic metabolism of fluorobenzene by Rhizobiales sp. strain F11 was investigated. Liquid chromatography-mass spectrometry analysis showed that 4-fluorocatechol and catechol were formed as intermediates during fluorobenzene degradation by cell suspensions. Both these compounds, unlike 3-fluorocatechol, supported growth and oxygen uptake. Cells grown on fluorobenzene contained enzymes for t...

Infusions of 2.5-7.8 ng. noradrenaline/Kg./min. over 30 min. and intruperitonenl injections of noradrenaline and adrenaline up to 2000 Mg/Kg. in the cat had no effect on the catechol amine content of the. heart, spleen, liver, kidney and skeletal muscle, except for a slight increase in the adrenaline content. It is concluded that these organs lack the ability to accumulate i-iirulating catechol...

Graphene based 2D nanomaterials have attracted increasing attention in biosensing application due to the outstanding physicochemical properties of graphene. In this work, palladium nanoparticles (Pd) loaded reduced graphene oxide (rGO) hybrid (rGO-Pd) was synthesized through a facile method. Laccase (Lac) was immobilized on rGO-Pd by utilizing the self-polymerization of dopamine, which generate...

Nature often serves as a model system for developing new adhesives. In aqueous environments, mussel-inspired adhesives are promising candidates. Understanding the mechanism of the extraordinarily strong adhesive bonds of the catechol group will likely aid in the development of adhesives. With this aim, we study the adhesion of catechol-based adhesives to metal oxides on the molecular level usin...

The Kms for esculetin and S-adenosyl-L-methionine for catechol O-methyltransferase from the yeast Candida tropicalis were 6.2 and 40 microM, respectively. S-Adenosyl-L-homocysteine was a very potent competitive inhibitor with respect to S-adenosyl-L-methionine, with a Ki of 6.9 microM. Of the catechol-related inhibitors, purpurogallin, with a Ki of 0.07 microM, showed the greatest inhibitory ef...

1. Two strains of Pseudomonas were grown with phenol and used to prepare cell extracts that metabolized catechol with the transient formation of 2-hydroxymuconic semialdehyde. 2. One of these preparations catalysed the conversion of 1mol. of catechol into 1mol. each of formate and 4-hydroxy-2-oxovalerate. 3. A method for the determination of 4-hydroxy-2-oxovalerate is described, together with s...

Tyrosinase has been immobilized on a Au nanoparticles encapsulated-dendrimer bonded conducting polymer on a glassy carbon electrode for the estimation of catechol. The modified electrode was characterized by cyclic voltammetry and AFM techniques. The principle of catechol estimation was based on the reduction of biocatalytically liberated quinone species at +0.2 V versus Ag/AgCl (3 M KCl), with...