Clathrin-coated vesicles (CCVs) mediate transport between the plasma membrane, endosomes and the trans Golgi network. Using comparative proteomics, we have identified coated-vesicle-associated kinase of 104 kDa (CVAK104) as a candidate accessory protein for CCV-mediated trafficking. Here, we demonstrate that the protein colocalizes with clathrin and adaptor protein-1 (AP-1), and that it is asso...

Chemoprophylaxis Vaccination (CVac) confers long lasting sterile protection against homologous parasite strains in humans, and involves inoculation of infectious sporozoites (SPZ) under drug cover. CVac using the drug chloroquine (CQ) induces pre-erythrocytic immunity in humans that includes antibody to SPZ and T-cell responses to liver stage (LS) parasites. The mechanism by which CVac with CQ ...

This paper describes a grapheme-to-phoneme conversion method using phoneme con-nectivity and CCV conversion rules with probabilistic phrase break detection. The method consists of mainly four modules including phrase-break detection, morpheme normalization, morpheme to phoneme conversion and phoneme connectivity check. In the experiments with a test corpus of 210 sentences, we achieved 85% of p...

During clathrin-mediated endocytosis, membrane scission marks the isolation of a cargo-laden clathrin-coated pit (CCP) from the cell exterior. Here we used live-cell imaging of a pH-sensitive cargo to visualize the formation of clathrin-coated vesicles (CCVs) at single CCPs with a time resolution of seconds. We show that CCPs are highly dynamic and can produce multiple vesicles in succession. U...

Color histograms are widely used for content-based image retrieval. Their advantages are eeciency, and insensitivity to small changes in camera viewpoint. However, a histogram is a coarse characterization of an image, and so images with very diierent appearances can have similar histograms. We describe a technique for comparing images called histogram re-nement, which imposes additional constra...

Loss of maternal UBE3A causes Angelman syndrome (AS), a neurodevelopmental disorder associated with severe epilepsy. We previously implicated GABAergic deficits onto layer (L) 2/3 pyramidal neurons in the pathogenesis of neocortical hyperexcitability, and perhaps epilepsy, in AS model mice. Here we investigate consequences of selective Ube3a loss from either GABAergic or glutamatergic neurons, ...

Accurate state of charge (SOC) estimation is essential for the whole-life-cycle safety guarantee and protection lithium-ion batteries, which quite difficult to realize. In this study, a novel weighting factor-adaptive Kalman filtering (WF-AKF) method proposed accurate SOC with collaborative model parameter identification. An improved bipartite electrical equivalent circuit (BEEC) constructed de...

Abstract In recent years, with the development of computer and network technologies, digital video image information is emerging more more, era based on multimedia services coming to us, people’s demand for visual media content such as images becoming extensive. this paper, we use Gaussian mixture model pixel detection technology method study meet various needs users. SVM used perform classific...

Adiposis dolorosa (AD) is a rare disorder of painful nodular subcutaneous fat accompanied by fatigue, difficulty with weight loss, inflammation, increased fluid in adipose tissue (lipedema and lymphedema), and hyperalgesia. Sequential compression relieves lymphedema pain; we therefore hypothesized that whole body cyclic pneumatic hypobaric compression may relieve pain in AD. To avoid exacerbati...