Sera from 31 Legionnaires' disease (LD) survivors of the Philadelphia outbreak, 31 Legionnaire (L) controls, and 300 additional controls were examined for the presence of specific antibodies to five antigen preparations of Legionella pneumophila (serogroup 1) to determine the effect of antigen preparation on the sensitivity and specificity of the indirect immunofluorescence test. Diagnostic lev...

O-glycans are suitable targets as novel and useful tumor markers. The structures of O-glycans in human sera from four healthy controls were precisely analyzed to obtain the reference O-glycan database. O-glycans were prepared from sera by hydrazine treatment followed by fluorescent labeling with aminopyridine and identified using two-dimensional mapping, enzymatic digestion and mass spectrometr...

The ability of monoclonal antibodies against the Thy-1.1 differentiation antigen to inhibit the growth of transplanted syngeneic AKR/J SL2 leukemic cells has been previously demonstrated. In the present study we further examined therapy with monoclonal antibody of the IgG2a isotype, which was the most effective isotype studied. Intravenous infusion of ascites fluid containing the anti-Thy-1.1 m...

It is common to characterize the spatial distribution of plant patterns as random, aggregate, or uniform. In this context, a major challenge for researcher choice method identify pattern correctly well factors related it. The vast literature on subject not recent, especially regarding dispersion indices. aim review was conduct critical and temporal analysis these indices test their effectivenes...

The expression of mammary tumor virus (MTV) antigen in the milk and various organs of three non-inbred dd mouse stocks (ddO, ddN and ddY) was examined by the immunodiffusion (ID) and micro-immunodiffusion (micro-ID) tests. The rate of MTV antigen expression in the milk was 100% at the first lactation in ddO (6/6) and ddN mice (10/10), and 23% in ddY mice (3/13). Mammary tumor incidence was 13% ...

The absolute frequencies of B cells-producing A5A idiotype have been determined in vitro by limiting dilution analysis in a culture system in which every LPS-reactive B cell grows into a clone of IgM-secreting cells. Spleen cells from normal A/J mice contain 1 A5A-idiotype-producing B-cell precursor in 2.5 X 10(3) LPS-reactive B cells. Approximately a 10-20-fold increase in frequencies of precu...

leptospirosis is one of the most prevalent zoonotic diseases worldwide. currently, multivalent whole-cell leptospiral vaccines can induce protection against leptospirosis. therefore, preparation and formulation of new generations of vaccines that can stimulate long-term immunity for leptospirosis control are essential. the aim of this study was to prepare and characterize alginate microspheres ...

the liver fluke fasciola hepatica causes fascioliasis, a liver disease in most part of the world and particularly in north of iran. diagnosis of the diseases is anchored in coprological manner but serological methods are preferable due to some obscurities. in this study, sera obtained from human patients infected with fasciola hepatica were tested by the enzymelinked immunotrotransfer blot (eit...