The kidney and several other thyroid hormone-responsive tissues contain a NADP-regulated thyroid hormone (TH)-binding protein (THBP), with an apparent molecular mass of 36 kDa on SDS-PAGE, responsible for most of the intracellular high-affinity T3 and T4 binding. THBP was purified to homogeneity from human kidney cytosol and used to generate proteolytic peptides. Microsequencing of four peptide...

We previously reported a cDNA selection method using DNA latex particles to identify expressed genes in specific regions of genomes and named this cDNA scanning method (Hayashida et al., 1995 Gene 155-161). We applied the cDNA scanning method to the YAC CIC3B1-S DNA on Arabidopsis thaliana chromosome 5, and constructed a region-specific sublibrary in which cDNAs for genes on the YAC CIC3B1S DNA...

We have been conducting a mouse cDNA project to predict protein-coding sequences of mouse homologues of human KIAA and FLJ genes since 2001. As an extension of these projects, we herein present the entire sequences of 500 mKIAA cDNA clones and 4 novel cDNA clones that were incidentally identified during this project. We have isolated cDNA clones from the size-fractionated mouse cDNA libraries d...

Nuclear import of viral cDNA is a critical step for establishing the proviral state of human immunodeficiency virus type 1 (HIV-1). The contribution of HIV-1 integrase (IN) to the nuclear import of viral cDNA is controversial, partly due to a lack of identification of its bona fide nuclear localization signal. In this study, to address this putative function of HIV-1 IN, the effects of mutation...

A new method for amplifying cDNA ends is described which requires only first-strand cDNA synthesis and a single PCR to generate a correct product with very low or no background. The method can be successfully applied to total RNA as well as poly A+ RNA. The same first-strand cDNA can be used to amplify flanking sequences of any cDNA species present in the sample.

1. The Importance of the Mouse Genome and Transcriptome Study 2. The History of Mouse Genetics 2.1. The Beginning of Mouse Genetics and the Establishment of Inbred Strains 3. The Determination of Mouse Genome Sequences 4. A Full-Length Mouse cDNA Project 4.1. From the EST Project to the Full-Length cDNA Project 4.2. Findings from Genome and cDNA Sequences 4.3. Functional Annotation of Mouse cDN...

The nucleotide sequence of a cDNA coding for the human acylamino acid-releasing enzyme (AARE, also known as acylpeptide hydrolase) [EC 3.4.19.1] subunit has been determined. The amino acid sequence of human AARE subunit deduced from its cDNA nucleotide sequence showed a high degree of identity (91.5%) with both the corresponding proteins from the pig and the rat. The AARE cDNA shows 99.2% ident...