T cell monitoring is increasingly performed using cryopreserved PBMC. It has been suggested that resting of PBMC after thawing, that is, culturing them overnight in test medium, produces higher antigen-induced spot counts in ELISPOT assays. To evaluate the importance of overnight resting, we systematically tested cryopreserved PBMC from 25 healthy donors. CEF peptides (comprising CMV, EBV and f...

We report the transport and thermodynamic properties under hydrostatic pressure in the antiferromagnetic Kondo compound CeMg2Cu9 with a two-dimensional arrangement of Ce atoms. Magnetic specific heat Cmag(T ) shows a Schottky-type anomaly around 30 K originating from the crystal electric field (CEF) splitting of the 4f state with the first excited level at ∆1/kB = 58 K and the second excited le...

Metabolic Pathway Analysis (MPA) is important tool in the field of metabolic engineering. The possible pathway from substrates to products, or internal loops could be analyzed by Elementary Mode (EM) or Extreme Pathways (EP). An application of EMs is to predict transcriptional patterns for carbon source perturbation conditions by Control Effective Fluxes (CEFs) [1]. In our previous study [2], C...

Although desirable for safety reasons, the host range restrictions of modified vaccinia virus Ankara (MVA) make it less applicable for general use. Propagation in primary chicken embryo fibroblasts (CEF) requires particular cell culture experience and has no pre-established record of tissue culture reproducibility. We investigated a variety of established cell lines for productive virus growth ...

The performance of a coherent rake receiver of DSCDMA systems is significantly affected by the accuracy of channel estimation. The performance of the channel estimator can be improved by employing a channel estimation filter (CEF) whose impulse response is adjusted according to the channel condition. In this paper, we consider the design of an adaptive channel estimator for DSCDMA systems with ...

Anaplasma marginale (A. marginale) is a worldwide pathogen that infects variety ofruminants, but mostly cattle. The present study aimed to describe an isolation technique for A. marginale, using chicken embryo Þ broblast (CEF) cell culture. Blood and tick samples were collected from 5 calves 2 3 months old, which considered be infected with A.marginale due anemia, jaundiced mucous membranes, pr...

This paper studies the event-triggered saturated adaptive iterative learning control (ETSAILC) problem for fractional-order multi-agent systems (FOMASs) subject to local Lipschitz nonlinearities and input saturation. First, a mechanism is proposed ensure that events occur along an iteration axis, all follower agents synchronously broadcast their states at each triggering step, receive restore i...

The angular distribution of 4f electrons in the presence of a crystal field has been derived by applying the Stevens operators formalism. For axial symmetry the progressive localization of the 4f electrons from the z-axis to the plane perpendicular may be mainly correlated with the variation of MJ (Mj = J, J 1, ...) according to the sign of the Stevens factor 03B1j. For cubic symmetry, due to t...

In Yemen, most of the medicines are imported. Drugs that enter country by legal means undergo some tests Supreme Board for and Medical Appliances (SBDMA) to evaluate quality product. However, illegally (smuggling) this makes them bypass evaluation their SBDMA. This work was carried out determine physical control parameters authorized smuggled drug products marketed in Aden pharmacies. The brand...