objective: spermatogonial stem cells (sscs) are the only cell type that can restore fertility to an infertile recipient following transplantation. much effort has been made to develop a protocol for differentiating isolated sscs in vitro. recently, three-dimensional (3d) culture system has been introduced as an appropriate microenvironment for clonal expansion and differentiation of sscs. this ...

objective: classic cell culture and karyotyping is routinely used for prenatal detection of different chromosomal abnormalities. molecular cytogenetic techniques have also recently been developed and used for this purpose. quantitative florescence pcr using short tandem repeat (str) markers has more potential for high throughput diagnosis. marker heterozygosity in short tandem repeats (str) is ...

Keloid, a tumor like scarring disorder, is caused by wounding in genetically predisposed individuals with poorly defined pathogenesis. To gain pathomechanistic details on keloid development, we performed single-cell RNA-sequencing and spatial transcriptomics edge adjacent non-lesional skin. Upon analysis, characterized ten major cell types based expression of known markers. Among those, fibrobl...

background: mature t/ nk-cell neoplasms are a rare group of disorders and their presentation as leukemia is even rarer. most of the previous studies have focused on mature b-cell lineage leukemias and there is a paucity of data on mature t/nk-cell lineage leukemias. we, therefore, planned this study to analyze their spectrum, frequency, morphology and immunophenotypic features. methods: all cas...

Abstract Mixed phenotype acute leukemia (MPAL) is an uncommon disease characterized by currently only limited knowledge concerning biology, clinical presentation, and treatment outcome. We here describe a most unusual case of simultaneous occurrence T-lymphoblastic lymphoma in cervical mediastinal lymph nodes myeloid the bone marrow (BM) successfully treated with allogeneic stem cell transplant...

Background: This study was conducted to identify and characterize repopulating spermatogonial stem cells (SSCs) in the adult human testes. Materials and Methods: Testes biopsies from obstructive azoospermic patients and normal segments of human testicular tissue were used. Flow cytometry, real time PCR and immunohistochemical analysis were performed. Purified human spermatogonia were transplant...

PCA–LDA scatter plot for Raman spectra of wild-type (circles) and radioresistant (traingles) breast cancer cell lines. An accuracy 100% is achieved in classifying from all 198 the test set (open markers).

objective: bone marrow mesenchymal stem cells reside in bone marrow and support homing and differentiation of hematopoietic stem cells (1). this makes them an excellent instrument for regenerative treatment and co-culture with the hsc. materials and methods: in this study, k562 cell lines were treated with butyric acid(for erythroid differentiation), co-cultured with mesenchymal stem cells and ...