نتایج جستجو برای: cell separation
تعداد نتایج: 1791062 فیلتر نتایج به سال:
Using staphylococci grown from single-cell isolations made at various stages of selection on penicillin media, it was observed that the 18 hr. progeny show variations in penicillin sensitivity, as tested by increase in resistance. The range of variation is about the same at any stage in selection; variation appears to be continuous in any one population of the microbes, since the number showing...
In this paper, we report an inertial microfluidic device with simple geometry for continuous extraction of large particles with high size-selectivity (<2 μm), high efficiency (∼90%), and high purity (>90%). The design takes advantage of a high-aspect-ratio microchannel to inertially equilibrate cells and symmetric chambers for microvortex-aided cell extraction. A side outlet in each chamber con...
In this paper a method is introduced to segment cytological stained cells in microscopic images with the histogram backprojection algorithm (HB). The segmented cell areas are classified in cell clusters and individual cells by a function based on iterative erosion. The cell cores are also segmented with the HB algorithm and the result is modified by image processing functions to get connected a...
the satellite cells. Contaminating CD45+/Mac-1+/Sca-1+ cells were subsequently removed by MACS cell separation. Cell cycle analysis revealed that over 80% of the resulting myogenic cell population was in G0, indicating a high proportion of quiescent cells in the isolated population. RT-PCR analysis showed that the expression of three Kruppel-like factors (Klf) was increased in quiescent satelli...
Cell separation technology is a key tool for biological studies and medical diagnostics that relies primarily on chemical labeling to identify particular phenotypes. An emergent method of sorting cells based on differential rolling on chemically patterned substrates holds potential benefits over existing technologies, but the underlying mechanisms being exploited are not well characterized. In ...
A glass slide covered with bacteria is pressed into another glass slide coated with partially cured polydimethylsiloxane (PDMS). The PDMS is hardened and the cells are removed to create a textured surface whose indentations preferentially capture the same type of bacteria when a mixture of bacteria is flowed over it. Overcoating the cell-imprinted PDMS with methylsilane groups causes the result...
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