Light and transmission electron microscopy are used in studying wall morphology and histochemical methods, including immunocytochemistry, can be used to locate specific compounds in walls. All plant cell walls contain a fibrillar phase of cellulose microfibrils and a matrix phase which contains a high proportion of non-cellulosic polysaccharides that vary in their chemical structures, depending...

CAMERON, C. M. & PURDOM, MARY, R. Immunological and chemical characteristics of Corynebacterium pseudotuberculosis cell walls and ptotoplasm. Onderstepoort]. vet. Res. 38 (2), 83-92 (1971). Successive extraction of purified Corynebacterium pseudotuberculosis cell walls with ether: ethanol, cold and hot trichloroacetic acid and crystalline trypsin did not destroy their immunizing potency. Cell w...

Activation of the alternate complement pathway in human serum by several bacterial components was compared. Peptidoglycan from group A streptococcal cell walls was the most active material, on a weight basis, followed by cell walls, protoplast membranes, and whole cells. The group-specific carbohydrate was inactive. Treatment of peptidoglycan with low concentrations of lysozyme or short periods...

Polysaccharide-rich cell walls are a defining feature of plants that influence cell division and growth, but many details of cell-wall organization and dynamics are unknown because of a lack of suitable chemical probes. Metabolic labeling using sugar analogs compatible with click chemistry has the potential to provide new insights into cell-wall structure and dynamics. Using this approach, we f...

The surveys of Cummins & Harris (1956, 1958) established that the cell walls of Gram-positive bacteria all contain the amino sugars glucosamine and muramic acid (3-O-a.-carboxyethylglucosamine) and the amino acids alanine, glutamic acid and either lysine or aci-diaminopimelic acid. In some species either glycine or aspartic acid was also present, whereas the other amino acids found in proteins ...

Maturation of potato (Solanum tuberosum L.) tuber native and wound periderm and development of resistance to periderm abrasion were investigated utilizing cytological and histochemical techniques. Both native and wound periderm consist of three different tissues: phellem, phellogen and phelloderm. It was previously determined that the phellogen walls of immature native periderm are thin and pro...

VEDROS, NEYLAN A. (Naval Medical Research Institute, Bethesda, Md.), AND PAUL R. HILL. Chemical and antigenic analysis of the cell walls of Neisseria meningitidis group B. J. Bacteriol. 91:1992-1997. 1966.-Cell walls have been isolated from Neisseria meningitidis group B, by rapid freeze-thawing or treatment with sodium deoxycholate. Chemical analysis of the cell walls indicated that the amino ...

Crown-gall tumor tissue cultures release peroxidase into the medium in response to the concentration of specific ions in the medium. This release is not due to diffusion from cut surfaces or injured cells. Calcium, magnesium, and ammonium were, in that order, most effective in increasing peroxidase release. The enzyme was demonstrated cytochemically on the cell walls and in the cytoplasm. Cell ...

It seems that 25 years ago plant cell walls were different than they are now. I don’t mean physically different, of course—they were made of the same old stuff—I mean conceptually different. Let me try to explain. Much of the plant body (and in large plants the bulk of it) is comprised of cell wall material. It forms a tough yet extensible extracellular matrix of polysaccharides for young and g...

Changes in neutral sugar, uronic acid, and protein content of tomato (Lycopersicon esculentum Mill) cell walls during ripening were characterized. The only components to decline in amount were galactose, arabinose, and galacturonic acid. Isolated cell walls of ripening fruit contained a water-soluble polyuronide, possibly a product of in vivo polygalacturonase action. This polyuronide and the o...