Eleven simple neutral water-miscible compounds were tested for protective action against freezing damage to human red blood cells. All the compounds penetrated the cells at room temperature without damage, but only four, N-substituted amides, were active. These results are considered together with previously published work on freezing protection by other low molecuar weight solutes. The affinit...

Isothermal titration calorimetry (ITC) is a straightforward method to determine basic chemical details of a binding interaction (affinity, thermodynamics and stoichiometry) in a single experiment and under native conditions [1, 2]. Traditionally, ITC experiments are performed using the method of incremental titration, whereby a precise volume of titrant is added to a solution of titrand at disc...

To address the molecular mechanism of T cell receptor (TCR) signaling, we have formulated a model for T cell activation, termed the 2D-affinity model, in which the density of TCR on the T cell surface, the density of ligand on the presenting surface, and their corresponding two-dimensional affinity determine the level of T cell activation. When fitted to T cell responses against purified ligand...

The reactions of propenyl magnesium halides with ethylene were studied using ab initio calculations. The structure of the transition state and the ground state were evaluated and obtained the structural properties, theoretical thermodynamic and kinetic data i.e. rate constants of the reactions. The methods used for calculations are RHF, B3LYP and MP2 with 6-31G* b...

Glycopeptides from the microplasmodia of Physarum polycephalum grown in the presence of [3H]mannose or [3H]glucosamine were characterized by gel-filtration, anion-exchange and lectin-affinity chromatography before and after enzymatic or chemical treatment. Various types of glycopeptide were found in the growing plasmodia as shown by Con A affinity column chromatography, while pulse-labeled prec...

The low-affinity interaction between human serum albumin (HSA) and Diclofenac sodium (DCF) was studied using NMR techniques. Both 13C-NMR chemical shift and linewidth show that the dichlorophenyl ring in DCF molecule plays a primary role in its interaction with HSA. Langmuir adsorption isotherm was applied to evaluate the association constant K and the number of binding sites n of the drug/HSA ...

This paper focuses on the effects of the addition of an ionic liquid, 1-Allyl-3-butylimidazilium bis(trifluoromethane sulfonyl)imide ([ABIM]TFSI), which has a high affinity for benzene, into the poly(vinyl chloride) (PVC) membrane on the pervaporation characteristics of the removal of benzene from aqueous solutions of dilute benzene. When aqueous solutions of 100～500 ppm benzene were permeated ...