BACKGROUND Osteoprotegerin (OPG) has been reported to prevent bone resorption by inhibiting the formation, function, and survival of osteoclasts in a variety of animal models. However, the effects of OPG on bone metabolism in avian species have not been described. The objective of this study was to investigate the effects of chicken OPG (chOPG) expressed in chicken embryo fibroblasts (CEFs) on ...

RNA sequencing transcriptome analysis using massively parallel next generation sequencing technology provides the capability to understand global changes in gene expression throughout a range of tissue samples. Development of the vertebrate retina requires complex temporal orchestration of transcriptional activation and repression. The chicken embryo (Gallus gallus) is a classic model system fo...

Inoculation of the Jensen rat sarcoma into the developing chick embryo gives a rapidly growing tumor at the site of inoculation, whether in the membranes or in the body of the chick itself. These tumors by transfer from embryo to embryo can be kept going for as long as forty-six days, and perhaps indefinitely in the foreign species. The rat cells show no morphological change even after a very l...

The potential of Sensi-1 Agrinak and KUB crossbred so-called SENKUB chicken as dual-purpose can be increased by selecting hatching egg weights to get DOC with good quality. study was conducted evaluate the grades eggs on production performances. complete randomized design applied in this experiment 3 treatments. treatments are (small 36-40 g, medium 41-45 large 46-50 g). process used two stages...

The observation and manipulation of chicken embryos in ovo has been useful for understanding the development vertebrates. However, viability longevity are severely compromised even by simple manipulations to egg shell. We have explored experimental protocols that promote ex use an undergraduate teaching laboratory setting. Here, we demonstrate a modified windowing technique increases survival t...

Summary Body axis elongation is a hallmark of the vertebrate embryo, involving architectural remodeling tail bud. Although it clear how neuromesodermal progenitors (NMPs) contribute to embryo elongation, dynamic events that lead de novo lumen formation and culminate in 3-dimensional, neural tube from NMPs, are poorly understood. Here, we used in vivo imaging chicken show ...

Hematopoietic stem cells (HSCs), which are responsible for blood cell production, are generated during embryonic development. Human and chicken embryos share features that position the chicken as a reliable and accessible alternative model to study developmental hematopoiesis. However, the existence of HSCs has never been formally proven in chicken embryos. Here, we have established a complete ...

With sequencing of the chicken genome largely completed, significant effort is focusing on gene annotation, including acquiring information about the patterns of gene expression. The chicken embryo is ideally suited to provide detailed temporal and spatial expression information through in situ hybridization gene expression analysis in vivo. We have developed the Gallus expression in situ hybri...

Not all vertebrates share the familiar system of XX:XY sex determination seen in mammals. In the chicken and other birds, sex is determined by a ZZ:ZW sex chromosome system. Gonadal development in the chicken has provided insights into the molecular genetics of vertebrate sex determination and how it has evolved. Such comparative studies show that vertebrate sex-determining pathways comprise bo...

Whole genome bisulfite sequencing (WGBS) analysis of DNA methylation uses massively parallel next generation sequencing technology to characterize global epigenetic patterns and fluctuations throughout a range of tissue samples. Development of the vertebrate retina is thought to involve extensive epigenetic reprogramming during embryogenesis. The chicken embryo (Gallus gallus) is a classic mode...