Jung-Seok Han, Kyung-Rae Dong, Yong-Soon Park, Woon-Kwan Chung*, Jae-Hwan Cho, and Young-Kuk Cho Depatment of Nuclear Medicine, Asan Medical Center, Seoul 138-736, Korea Department of Radiological Technology, Gwangju Health College University, Gwangju 506-701, Korea Department of Nuclear Engineering, Chosun University, Gwangju 501-759, Korea 4 Department of International Radiological Science, H...

A mammalian endonuclease that resolves Holliday junctions has been partially purified from extracts of calf thymus and Chinese hamster ovary cells. The activity acts upon (i) synthetic Holliday junctions and (ii) recombination intermediates made by the Escherichia coli RecA protein and appears to be functionally analogous to the E. coli RuvC protein. Cleavage occurs by the introduction of symme...

To examine the role of early carbohydrate recognition/trimming reactions in targeting endoplasmic reticulum (ER)-retained, misfolded glycoproteins for ER-associated degradation (ERAD), we have stably expressed the cog thyroglobulin (Tg) mutant cDNA in Chinese hamster ovary cells. We found that inhibitors of ER mannosidase I (but not other glycosidases) acutely suppressed Cog Tg degradation and ...

facilitates apoptosis by targeting several anti-apoptotic genes. If miR-466h-5p activation is demonstrated to be a universal early response in apoptotic pathways in CHO, 2 Mü ller, D. et al. (2008) MicroRNAs as targets for engineering of CHO cell factories. Trends Biotechnol. 26, 359–365 3 Xu, X. et al. (2011) The genomic sequence of the Chinese hamster ovar (CHO)-K1 cell line. Nat. Biotechnol....