Thermal denaturation of protonated polynucleotides is accompanied by a change in protonation [l-5] . The thermal transition at a given pH can thus be directly related to the pk’ of the proton-induced structural transition at a given temperature. For poly (A’).poly (A+) a strict relationship has been shown to hold over a large range of conditions [4-61. In fig. 1 the results in the literature an...

Many DNA lesions cause pausing of replication forks at lesion sites thus generating gaps in the daughter-strands that are filled-in by post replication repair (PRR) pathways. In S. cerevisiae, PRR involves Translesion Synthesis (TLS) mediated by Polη or Polζ, or Rad5-dependent gap-filling via a poorly characterized error-free mechanism. We have developed an assay to monitor error-free and mutag...

Purpose TNF inhibitors are established treatment for poly-juvenile idiopathic arthritis (pJIA), but there are needs of alternative therapy for refractory patients. Tocilizumab (TCZ) has been widely used for rheumatoid arthritis (RA) in adults, and the effectiveness for systemic JIA has been well-documented. However, the experience on pJIA is limited. We compared the clinical courses of our pati...

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We have measured the temperature driven denaturing, or melting transition in poly d(A)-poly d(T) DNA oligomers of various lengths in different buffer conditions. Our findings are in clear disagreement with two state, reaction kinetics model, and we find that the so-called zipper model, where denaturing proceeds through opening of the duplex at the ends describes well the temperature dependence ...

Wide distribution of chlorinated paraffins in the environment has already been demonstrated in several studies; however, information about their levels in the Central Europe is still very limited. First study focused on the SCCP contamination of the Czech aquatic environment have been performed recently, and its results motivated the authors to analyze sediments from a wide set of the Czech riv...