a simple, green and efficient method for synthesis of 2,4,5-trisubstituted imidazoles is described via three-component cyclocondensation of benzil or benzoin, aldehyde and ammonium acetate by using the nano sio2-supported ferric hydrogen sulfate (fhs/sio2), as a catalyst, under solvent-free conditions. the nano sio2 support and catalyst were characterized by x-ray diffraction (xrd), transmissio...

A simple, selective, accurate high performance liquid chromatographic method was developed and validated for an analysis of Halobetasol Propionate and p-Chlorocresol in pharmaceutical formulations. Chromatographic separation achieved by gradient programme on a C18 column (Waters Novapak C-18, 250 x 4.6mm, 4μm column) utilizing a mobile phases of A (pH 2.5 phosphate buffer) and B (mixture of buf...

A simple, specific, precise and accurate reversed phase liquid chromatographic (RP-LC) method has been developed for the determination of ascorbic acid in different food additives. The chromatographic separation was achieved on a LiChrosorb C18, 250 mm x 4.6 mm, 5 μm column at a detector wavelength of 230 nm and a flow rate of 1.5 ml/min. The mobile phase was composed of acetonitrile and water ...

PURPOSE A simple stability indicating reverse phase liquid chromatographic method was developed for the simultaneous determination of rosuvastatin and ezetimibe in pharmaceutical formulations. METHODS Best chromatographic response was achieved with C18 column (250 X 4.6 mm, 5µm) with photo diode array (PDA) detector. The mobile phase was composed of a mixture of sodium acetate buffer (pH 4.0)...

The most suitable bio-analytical method based on liquid-liquid extraction has been developed and validated for quantification of Rasagiline in human plasma. Rasagiline-13C3 mesylate was used as an internal standard for Rasagiline. Zorbax Eclipse Plus C18 (2.1 mm×50 mm, 3.5 μm) column provided chromatographic separation of analyte followed by detection with mass spectrometry. The method involved...

A new high-performance liquid chromatographic method is described for the determination of bisoprolol in human plasma. The proposed method was based on the derivatization of bisoprolol with 4-chloro-7-nitro-2,1,3-benzoxadiazole in borate buffer at pH 9.5 to yield a fluorescent product. Chromatographic separation of bisoprolol was achieved by using isocratic elution at a flow rate of 1.2 mL/min ...

A new analytical methodology for a simple and efficient on-line preconcentration of trace inorganic anions in water and salt samples prior to ion chromatographic determination is proposed. The preconcentration method is based on partition/ion-exclusion chromatographic ion stacking (PIEC ion stacking) with a hydrophilic polymer gel column containing a small amount of fixed anionic charges. The d...

The analytical evaluation is described of a [125I]-aldosterone radioimmunoassay method for measuring aldosterone in human plasma, which requires no chromatographic purification before quantification by radioimmunoassay but does require organic solvent extraction. Rabbit anti-aldosterone serum is used, generated against aldosterone-3-oxime coupled to thyroglobulin. The antibody demonstrates negl...

An analytical method based on solid phase extraction has been developed and validated for analysis of amoxicillin in human plasma using gemifloxacin as an internal standard. A COSMOSIL 5C18-PAQ column provided chromatographic separation of analytes followed by detection with mass spectrometry. The method involves simple isocratic chromatographic conditions and mass spectrometric detection in th...

In this work, a validated stability-indicating ultra-high performance liquid chromatographic (UPLC) method has been developed for quantitative determination of related substances in Risperdal Consta. The chromatographic separation was achieved on a Waters BEH C18 column (2.1 × 100 mm, 1.7 μm) in an isocratic elution mode. The limit of quantification is 0.1 μg/mL, and the method is linear over t...