For more than two decades, chromosome aberration analysis has been used in biomonitoring of occupational and environmental exposure to ionising radiation. Chromosome aberration analysis is a method used to detect unstable aberrations in the lymphocytes of irradiated personnel. In turn, stable chromosome aberrations that arise some time after exposure are detected using the multicolour fluoresce...

Repeated DNAs from the constitutive heterochromatin of human chromosomes 1 and 18 were used as probes in nonradioactive in situ hybridization experiments to define specific numerical and structural chromosome aberrations in three human glioma cell lines and one neuroblastoma cell line. The number of spots detected in interphase nuclei of these tumor cell lines and in normal diploid nuclei corre...

The estrogen receptor (ER) gene is located on chromosome 6. The aim of our study was to investigate whether numerical chromosomal aberrations were reflected in estrogen/progesterone receptor (PgR) status and staining pattern. Fine-needle aspirates from 51 breast carcinomas were investigated immunocytochemically for ER/PgR and by in situ hybridization technique using digoxigenin-labeled alpha-sa...

Paraffin-embedded tissue samples from 30 cases of non-Hodgkin's lymphoma(NHL) and 10 of reactive hyperplasia, were processed for interphase cytogenetic chromosomal study. We performed non-fluorescent in situ hybridization(NFISH) using the enzymatic method with digoxigenin-labeled DNA centromeric probes for chromosome 7,12,18 and X, and a painting probe for chromosome 18. Chromosomal aberrations...

BACKGROUND Radiation-induced chromosome aberrations lead to a plethora of detrimental effects at cellular level. Chromosome aberrations provide broad spectrum of information ranging from probability of malignant transformation to assessment of absorbed dose. Studies mapping differences in radiation sensitivities between human chromosomes are seldom undertaken. Consequently, health risk assessme...

background: cytogenetic study of reproductive wastage is an important aspect in determining the genetic background of early embryogenesis. approximately 15 to 20% of all pregnancies in humans are terminated as recurrent spontaneous abortions (rsas). the aim of this study was to detect chromosome abnormalities in couples with rsas and to compare our results with those reported previously. materi...

Genotoxic agents induce chromosomal alterations, such as aberrations, micronuclei, and sister chromatid exchanges as well as mutations both in vivo and in vitro. Ionizing radiation and typical radiomimmetic agents such as bleomycin are very efficient inducers of chromosomal aberrations. The type of aberrations induced by these agents are cell-cycle dependent, i.e., chromosome type in pre-replic...

between the cytotoxic effects of these agents and the incidence of chromosome aberrations in the treated cells (2,4, 15). However, BLM treatment severely inhibits the progression of cells into mitosis. The probability that the extensively damaged cells might not be reaching mitosis makes it difficult to assay the chromosome damage accurately by the conven tional techniques. Recently, we have sh...