نتایج جستجو برای: chromosome specific microsatellite markers throughout this study
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the japanese and english white quails are widespread strains and belongs to the galliformes order, phasianidae family, coturnix genus and japonica species. these birds are likely to be well-adapted to the hard conditions and resistance to diseases as it has attained economic importance as an agricultural species. in the current study, the genetic variation of japanese and english white quail ...
We constructed a microsatellite library from four Crocidura russula Y chromosome-specific bacterial artificial chromosome (BAC) clones. Only one of eight microsatellites was male-specific, despite genome walking to obtain more flanking sequence and testing of 93 primer combinations. Potential reasons for this low success are discussed. The malespecific locus, CRY3, was genotyped in 90 males, in...
nuclear markers such as microsatellites have allowed the identification of conservation and management populations of the hawksbill turtles. in present study, eight microsatellite loci were studied. 60 samples of hawksbill turtles. flipper from shidvar and hormuz islands have been surgically removed and preserved in 20% dmso buffer. dna was extracted using dnp kit and amplified by pcr methods. ...
The study of microsatellite instability (MSI) has provided the evidence to support a sequential, progressive pathway for the development of cancer. In this study, we analyzed the role of MSI at chromosome 11p15.5 using microdissection of paraffin-embedded tissue from 68 matched normal and breast tumor samples. Components of intraductal, invasive and metastatic foci in lymph node were assessed f...
Quantitative trait loci affecting milk yield, health, and type traits were studied for seven large U.S. Holstein grandsire families using the granddaughter design. Seventy microsatellite markers located throughout the genome were selected for the quantitative trait loci scan. Effects of marker alleles were analyzed for 30 traits (21 type traits, 5 milk traits, 2 calving ease triats, and somatic...
Allelic association methods based on increased transmission of marker alleles will have to be employed for the mapping of complex disease susceptibility genes. However, because the extent of association of single marker alleles with disease is a function of the relative frequency of the allele on disease-associated chromosomes versus non disease-predisposing chromosomes, the most associated mar...
We have analyzed five microsatellite loci from the nonrecombining portion of the human Y chromosome in 15 diverse human populations to evaluate their usefulness in the reconstruction of human evolution and early male migrations. The results show that, in general, most populations have the same set of the most frequent alleles at these loci. Hypothetical ancestral haplotypes, reconstructed on th...
Previous allelotyping studies of epithelial ovarian carcinoma suggest that loss of heterozygosity on chromosome 14q may be a common genetic alteration in this tumor type. The purpose of this study was to determine a precise frequency of chromosome 14q allelic loss in ovarian carcinomas and to define a minimal region(s) of deletion. Seventy-six ovarian carcinomas representative of the complete s...
Marker-assisted backcross breeding program was anticipated to introgress bacterial blight (BB) resistance genes into an elite fine-grain rice cultivar VGD 1. Pyramided with three (xa5, Xa13, Xa21), ADT 55 the donor parent. A parental polymorphism survey executed between VGD1 and using genome-wide simple sequence repeats (SSR) also called microsatellite markers, identify polymorphic markers. Dis...
An increased plasma triglyceride (TG) level is associated with coronary artery disease (CAD) and myocardial infarction (MI) and is a key characteristic of the metabolic syndrome. Here, we used a genome-wide linkage scan to identify a novel genetic locus that influences the plasma TG level. We genotyped 714 persons in 388 multiplex Caucasian families with premature CAD and MI with 408 polymorphi...
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