Herein we report the possibility of using green and red emitting silica-coated cadmium selenide (CdSe) quantum dots (QDs) for remarkable stem and cancer cellular imaging, efficient cellular uptake and fluorescence imaging of semi and ultra-thin sections of tumor for in vivo tumor targeted imaging applications. The comparative studies of high contrast cellular imaging behaviours of the silica-co...

A facile approach for producing fluorescent composite paper containing nitrogen-doped graphene quantum dots (N-GQDs) and graphene on the surface of the modified fibers was implemented from the exfoliation of graphite oxide (GO) using a one-step hydrothermal method. The properties of the composite paper were characterized by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), scanni...

Developments in imaging technology now enable visualization of the functioning of individual ion channels in living cells: something previously possible only by the electrophysiological patch-clamp technique. We review techniques that track channel gating via changes in intracellular [Ca2+] resulting from openings of Ca(2+)-permeable channels. Spatial and temporal resolution are optimized by mo...

In situ pigment contents of biofilm-dwelling bdelloid rotifers of the Garonne River (France) were measured by high performance liquid chromatography (HPLC) and compared with pigment composition of surrounding biofilm microphytobenthic communities. Among pigments that were detected in rotifers, the presence of carotenoids fucoxanthin and myxoxanthophyll showed that the rotifers fed on diatoms an...

Interference by autofluorescence is one of the major shortcomes of immunofluorescence analysis by confocal laser scanning microscopy (CLSM). CLSM requires minimal tissue autofluorescence and reduced unspecific fluorescence background, requisites that become more critical when direct immunofluorescence studies are concerned. To control autofluorescence, different reagents and treatments can be u...

During this study, microtechnology and plasma etching were used to produce gratings 1.0 (TiD01), 2.0 (TiD02), 5.0 (TiD05), and 10.0 microns wide (TiD10) into commercially pure titanium wafers. After incubation of rat dermal fibroblast (RDFs) on these surfaces for 3 days, the cells were observed with scanning electron (SEM), transmission electron (TEM), and confocal laser scanning microscopy (CL...

We present an accuracy evaluation of a semi-automatic registration technique for 3D volume reconstruction from fluorescent confocal laser scanning microscope (CLSM) imagery. The presented semi-automatic method is designed based on our observations that (a) an accurate point selection is much harder than an accurate region (segment) selection for a human, (b) a centroid selection of any region i...

BACKGROUND Early exposure of infants and long-term immunity suggest that colonization with Moraxella catarrhalis is more frequent than is determined by routine culture. We characterized a reservoir of M. catarrhalis in pharyngeal lymphoid tissue. METHODS Tissue from 40 patients (median age, 7.1 years) undergoing elective tonsillectomy and/or adenoidectomy was analyzed for the presence of M. c...