Pseudomonas aeruginosa is able to grow on acyclic monoterpenes (citronellol, citronellate, geraniol and geranylate), and on other methyl-branched compounds such as leucine or isovalerate. The catabolic pathway of citronellol (Atu, acyclic terpene utilization) enters that of leucine/isovalerate (Liu, leucine and isovalerate utilization) at the level of methylcrotonyl-CoA. Key enzymes of the comb...

With more than 1.4 billion overweight or obese adults worldwide, obesity and progression of the metabolic syndrome are major health and economic challenges. To address mechanisms of obesity, adipose tissue-derived mesenchymal stem cells (ADSCs) are being studied to detail the molecular mechanisms involved in adipogenic differentiation. Activation of the Wnt signalling pathway has inhibited adip...

of medium chain fatty acids (MCFAs; C 8 –C 12) requires their activation inside peroxisomes by an acyl-CoA syn-SFB Biomembrane Research Center Institut fü r Biochemie und Lebensmittelchemie thetase, Faa2p. Myristoylation of membrane-associated proteins is catalyzed by Nmt1p (for review on lipid syn-Technische Universitä t Graz Petersgasse 12 thesis in yeast, see Kohlwein et al., 1996). S. cerev...

In isolated hepatocytes prepared from unfed neonatal chicks, stimulation of fatty acid synthesis by fructose or dihydroxyacetone required acetate or octanoate in the medium, suggesting that the stimulatory effect of these compounds required the enhanced production of intramitochondrial acetyl coenzyme A. Stimulation of fatty acid synthesis by lactate or pyruvate did not require a supplementary ...

The AMP-activated protein kinase is responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It may also regulate cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. We have purified the AMP-activated protein kinase 14,000-fold from porcine liver. The 63-kDa catalytic subunit ...

We have characterized the expression of potential acetyl-CoA-generating genes (acetyl-CoA synthetase, pyruvate decarboxylase, acetaldehyde dehydrogenase, plastidic pyruvate dehydrogenase complex and ATP-citrate lyase), and compared these with the expression of acetyl-CoA-metabolizing genes (heteromeric and homomeric acetyl-CoA carboxylase). These comparisons have led to the development of testa...

Previous studies in rats and humans have demonstrated that acetyl-CoA carboxylase alpha (ACC-alpha), the principal ACC isoenzyme in lipogenic tissues, is transcribed from two promoters, PI and PII, that operate in a tissue-specific fashion. Each promoter gives rise to ACC-alpha mRNA isoforms that differ in their 5' untranslated regions but essentially encode the same protein product. In the pre...

BACKGROUND Hypothalamic AMPK acts as a cell energy sensor and can modulate food intake, glucose homeostasis, and fatty acid biosynthesis. Intrahypothalamic fatty acid injection is known to suppress liver glucose production, mainly by activation of hypothalamic ATP-sensitive potassium (K(ATP)) channels. Since all models employed seem to involve malonyl-CoA biosynthesis, we hypothesized that acet...

Treatment of rat epididymal adipose tissue with insulin for a few minutes leads to a substantial increase in the rate of fatty acid synthesis, which is explained in part by rapid activation of the cytoplasmic enzyme acetyl-CoA carboxylase (Denton, 1975; Denton et al., 1977; Brownsey & Denton, 1979). The effects of insulin on fatty acid synthesis are opposed by adrenaline, but the changes in kin...