Macrolide and lincosamide (ML) resistance and the related resistance genes of staphylococci were assessed from cases of bovine subclinical mastitis. Of the 104 Staphylococcus aureus and 62 coagulase negative staphylococcus (CoNS) isolates, 26 (25%) and 12 (19.4%) were resistant to ML, respectively. While constitutive ML resistance phenotype accounted for 15.4% (16/104) of S. aureus and 8.1% (5/...

PURPOSE We evaluated the safety of saving partially used syringes of glutaraldehyde cross-linked collagen for subsequent treatment sessions in an individual. MATERIALS AND METHODS After periurethral injection in an office setting 56 partially used syringes of glutaraldehyde cross-linked collagen were stored in a refrigerator for 1 to 61 weeks (mean 15). Collagen from all 56 syringes was then ...

AIM To assess whether the information provided by automated continuous monitoring blood culture systems could aid in the diagnosis of catheter related sepsis. METHODS Serial dilutions of a strain of coagulase negative staphylococcus were inoculated into the BacT/Alert blood culture system. Blood culture results for seven patients with possible catheter related sepsis from coagulase negative s...

BACKGROUND Bacteria are the major contributor of ocular infections worldwide. Ocular infections, if left untreated, can damage the structures of the eye with possible blindness and visual impairments. This work was aimed to review the bacterial profile of ocular infections. METHODS Literature search was made in different electronic databases; the review was systematically made to get concrete...

The binding of staphylococcal components to fibrinogen was studied. Fibrinogen-binding material from lysed staphylococcal cells or culture supernatants was affinity purified on fibrinogen-Sepharose and analyzed on Western (immuno-) blots by the use of fibrinogen and antifibrinogen antibodies. Two main bands of 87 and 19 kilodaltons (kDa) and a weaker band of 35 kDa bound specifically to fibrino...

Objective: Isolation and determine antibiotic sensitivity test for coagulase positive Staphylococcus aureus isolates from restaurants and fast food serving hotels from Lusaka, Zambia. Methods: A total of 205 Paper currency notes of Zambia Kwacha (50,100,500,1000,5000,10,000,20,000 and 50,000)were randomly collected from market place and swab samples were analyzed in microbiology lab for S.aureu...

WHEN. in a routine laboratory, large numbers of cultures have to be made, many of them are incubated late in the afternoon and examined the next morning. When colonies of Staphylococcus aureus grow after a short incubation period few produce pigment, and it is impossible to say definitely if they are, in fact, Staph. aureus or Staph. albus. It is usual to test these organisms for coagulase but ...

OBJECTIVE To evaluate the presence of microorganisms of the genus Staphylococcus and Streptococcus on toys in the playroom of a teaching hospital, as well to as analyze the antimicrobial from the isolated strains. METHODS Samples were collected from 60 toys, using wet swabs, soon after being used by the children. The samples were inoculated in enriched and selective agar for isolation and lat...

The aim of the study was to characterise the free coagulase-negative Staphylococcus sp. strains isolated from normal (9.4%) and inflamed quarter milk samples of dairy cows in one herd localised in southern Poland. The isolates were examined phenotypically by cultural features, tube coagulase test, clumping factor (CF), and commercial agglutination test. Total number of 427 strains were cultivat...

Many of the commercial slide agglutination tests for Staphylococcus aureus incorporate antibodies against cell surface antigens associated with methicillin resistance, including capsular polysaccharides and an uncharacterized antigen, serotype 18. These tests are more sensitive than the first-generation agglutination procedures that detected only bound coagulase and protein A, but they suffer f...