Multiplex polymerase chain reaction (PCR) strategy is described for rapid identification of clinically relevant methicillin resistant Staphylococcus aureus (MRSA) that targets mecA and coagulase genes. In this study, 150 staphylococcal clinical isolates were used that included 40 isolates of MRSA, 55 isolates of methicillin susceptible S. aureus (MSSA), 44 isolates of methicillin susceptible co...

AIMS To ascertain the clinical importance of a strain of slide coagulase positive but tube coagulase negative Staphylococcus species isolated from the blood culture of a 43 year old patient with refractory anaemia with excessive blasts in transformation who had neutropenic fever. METHODS The isolate was investigated phenotypically by standard biochemical methods using conventional biochemical...

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of rabbit immunoglobulin G (IgG) antibodies to purified cell wall teichoic acids from the Staphylococcus aureus Lafferty strain and three strains of coagulase-negative staphylococci. Significant immunological cross-reactivity occurred only between the teichoic acid of S. aureus and one coagulase-negative preparation. T...

OBJECTIVE To evaluate the presence of microorganisms of the genus Staphylococcus and Streptococcus on toys in the playroom of a teaching hospital, as well to as analyze the antimicrobial from the isolated strains. METHODS Samples were collected from 60 toys, using wet swabs, soon after being used by the children. The samples were inoculated in enriched and selective agar for isolation and lat...

determination of the frequency of bacteria isolated from blood culture and the pattern of their antibiotic sensitivity is epidemiologically of great importance and can help choosing primary antimicrobial treatment. the aim of the current study was to determine the most common bacterial factors of blood infection and their antibiotic sensitivity in a one year period from october 2010 till the en...

this is a report of a case study on 234 patients with impetigo who referred to razi dermatology hospital from april to november, 1989. treatment was started immediately after obtaining direct smear and performing culture and antibiotic sensitivity test. the most common organism responsible for impetigo was the coagulase-positive staphylococcus (71%). in 13.7% of the cases, the coagulase-negativ...