نتایج جستجو برای: cobas amplicor monitor test

تعداد نتایج: 883872  

Journal: :Journal of clinical microbiology 1998
M Puolakkainen E Hiltunen-Back T Reunala S Suhonen P Lähteenmäki M Lehtinen J Paavonen

The diagnostic performance of a PCR test (Roche Cobas Amplicor CT/NG Test) and that of a ligase chain reaction (LCR) test (Abbott LCx Chlamydia trachomatis assay) were compared by using endocervical and urethral swab specimen culture as a reference test. First-void urine (FVU) and endocervical and urethral swab specimens were collected from 1,015 unselected patients attending a sexually transmi...

Journal: :The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease 2011
S Mitarai M Okumura E Toyota T Yoshiyama A Aono A Sejimo Y Azuma K Sugahara T Nagasawa N Nagayama A Yamane R Yano H Kokuto K Morimoto M Ueyama M Kubota R Yi H Ogata S Kudoh T Mori

OBJECTIVE A new loop-mediated isothermal amplification (LAMP) test kit, including a simple DNA extraction device for the detection of Mycobacterium tuberculosis complex, was developed for commercial use and evaluated for its usefulness in diagnosing tuberculosis (TB). DESIGN The LAMP test was performed using untreated and N-acetyl-L-cysteine (NALC) NaOH-treated sputum specimen. The efficiency...

2013
Alexander Jenson Laura Dize Harran Mkocha Beatriz Munoz Jennifer Lee Charlotte Gaydos Thomas Quinn Sheila K. West

PURPOSE To determine the sensitivity, specificity, and field utility of the Cepheid GeneXpert Chlamydia trachomatis (CT) Assay (GeneXpert) for ocular chlamydia infection compared to Roche Amplicor CT assay (Amplicor). METHODS In a trachoma-endemic community in Kongwa Tanzania, 144 children ages 0 to 9 were surveyed to assess clinical trachoma and had two ocular swabs taken. One swab was proce...

Journal: :Journal of clinical microbiology 2014
Miao Cui Nicholas Chan Momo Liu Khanh Thai Joanna Malaczynska Ila Singh David Zhang Fei Ye

Evaluation of the Cobas 4800 test demonstrated that Cobas had a low rate of cross-reactivity with low-risk human papillomavirus (lrHPV), a 3.74% disconcordance rate between prealiquots and postaliquots, and failure rates of 4.57% and 1.16%, respectively, after vortexing and swirling. This study demonstrated that the Cobas test has good sensitivity, accuracy, and reproducibility for detecting 14...

Journal: :Acta dermatovenerologica Alpina, Pannonica, et Adriatica 2005
M Poljak K Fujs K Seme B J Kocjan E Vrtacnik-Bokal

BACKGROUND Persistent infection with a subgroup of at least 15 high-risk human papillomavirus (HPV) genotypes is considered as a necessary although insufficient etiological factor in the development of cervical carcinoma. As a consequence, HPV testing has recently become an important part of the cervical carcinoma screening and detection algorithms. AIM OF THE STUDY To evaluate the analytical...

Journal: :Journal of clinical microbiology 2005
Maaike A P C van Ham Judith M J E Bakkers Gonneke K Harbers Wim G V Quint Leon F A G Massuger Willem J G Melchers

Certain high-risk (HR) human papillomavirus (HPV) types are a necessary cause for the development of cervical disorders. Women with persistent HR HPV infections have an increased risk of developing high-grade cervical lesions, compared with those who have no or low-risk HPV infections. Therefore, implementation of HPV detection into cervical screening programs might identify women at risk of ce...

Journal: :Journal of clinical microbiology 1998
A Bonington J I Strang P E Klapper S V Hood W Rubombora M Penny R Willers E G Wilkins

Several nucleic acid-based amplification tests are available for the detection of Mycobacterium tuberculosis, but few data are available on their use in the diagnosis of tuberculous meningitis (TBM). We performed a prospective study to assess the Roche AMPLICOR Mycobacterium tuberculosis PCR test (TB AMPLICOR) for use in the diagnosis of TBM and compared it with direct Ziehl-Neelsen staining of...

Journal: :Journal of clinical microbiology 2003
Xiaoli L Pang Linda Chui Jayne Fenton Barbara LeBlanc Jutta K Preiksaitis

In order to evaluate the LightCycler-based PCR (LC-PCR) as a diagnostic assay technique, a classical pp65 antigenemia assay and the commercially available COBAS Amplicor CMV Monitor (CACM) assay were compared to the LC-PCR assay for the detection and quantitation of cytomegalovirus (CMV) load in 404 parallel specimens of peripheral blood from 66 patients after solid organ transplantation. A goo...

2013
Soomin Ahn Jeeyun Lee Ji-Youn Sung So Young Kang Sang Yun Ha Kee-Taek Jang Yoon-La Choi Jung-Sun Kim Young Lyun Oh Kyoung-Mee Kim

BACKGROUND Recently, BRAF inhibitors showed dramatic treatment outcomes in BRAF V600 mutant melanoma. Therefore, the accuracy of BRAF mutation test is critical. METHODS BRAF mutations were tested by dual-priming oligonucleotide-polymerase chain reaction (DPO-PCR), direct sequencing and subsequently retested with a real-time PCR assay, cobas 4800 V600 mutation test. In total, 64 tumors includi...

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