The acid solubility of Type I collagen from rat tail tendons decreases due to diabetes. This finding has been taken as evidence that collagen from diabetics may be more cross-linked than normal. We compared CNBr peptide maps prepared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis for [3H] NaBH4-reduced tail tendons from streptozotocin-diabetic rats with maps from age-matched contr...

Rhabdomyosarcoma (RMS) is the most common soft-tissue sarcoma of childhood with a propensity to metastasize. Current treatment for patients RMS includes conventional systemic chemotherapy, radiation therapy, and surgical resection; nevertheless, little no improvement in long term survival has been achieved decades—underlining need target discovery new therapeutic approaches targeting tumor cell...

The concentrations of NaB3H4-reducible collagen cross-links were determined at the time when collagen fibres and bundles are observed in electron micrographs of connective tissue developing around the implanted Ivalon sponge in adult male rats. The highest radioactivity occurs with hydroxylysinonoreleucine and histidinohydroxymerodesmosine, and the lowest with lysinonorleucine, the reducible am...

OBJECTIVE In the United States, around 50% of all musculoskeletal injuries are soft tissue injuries including ligaments and tendons. The objective of this study is to assess the role of amnion-derived cellular cytokine solution (ACCS) in carboxy-methyl cellulose (CMC) gel in the healing of Achilles tendon in a rat model, and to examine its effects on mechanical properties and collagen content. ...

Aminoguanidine (AG) is an inhibitor of protein modification by the advanced Maillard reaction. We evaluated its effects in preventing age-related collagen cross-linking, glycation, and glycoxidation in Fischer 344 rats by administering the drug in their drinking water at 1 g/l from the time they were 6 months until they were 24 months of age. Body weight and food and water consumption were cons...

Digestion of preparations of insoluble collagen of guinea fraction, “soluble collagen,” decreased with the age of the animal (2) have led to a considerable interest in the chemical basis of this “aging” phenomenon. The concept of the progressive formation, extracellularly, of covalent cross-links in collagen fibrils has recently received direct support from the reports of Bornstein, Kang, and P...

Lysyl hydroxylase 2 (LH2) catalyzes the hydroxylation of lysine residues in the telopeptides of fibrillar collagens, which leads to the formation of stable collagen cross-links. Recently we reported that LH2 enhances the metastatic propensity of lung cancer by increasing the amount of stable hydroxylysine aldehyde-derived collagen cross-links (HLCCs), which generate a stiffer tumor stroma (Chen...

There has been considerable focus in investigations on the delivery systems and clinical applications of bone morphogenetic protein‑2 (BMP‑2) for novel bone formation. However, current delivery systems require high levels of BMP‑2 to exert a biological function. There are several concerns in using of high levels of BMP‑2, including safety and the high cost of treatment. Therefore, the developme...

The collagens were studied in 13 normal and 19 myxomatous human mitral valves. The collagens of the valve were completely solubilized by using a method consisting of guanidinium chloride extraction, limited pepsin digestions and CNBr cleavage of the residue. The normal valves contained 74% type I, 24% type III and 2% type V collagen. The type I and type III collagens had similar solubility patt...

The skin of Helix pomatia was investigated by biochemical methods and electronmicroscope. Biochemical investigations showed that Helix pomatia collagen represents a methionine-lacking collagen resistant to CNBr-cleavage. Electron microscopic studies showed a cross striation pattern of 53--57 nm.