An estimated 12 million persons throughout the world suffer from the protozoan disease leishmaniasis. Current treatments have liabilities including poor activity against some forms of leishmaniasis, toxicity, or the need for parenteral administration. Higher throughput methods to screen chemical compounds are needed to facilitate the search for new antileishmania drugs. In the mammalian host, L...

BACKGROUND Despite a significant increase in the number of patients with paracetamol poisoning in the developing world, plasma paracetamol assays are not widely available. The purpose of this study was to assess a low-cost modified colorimetric paracetamol assay that has the potential to be performed in small laboratories with restricted resources. METHODS The paracetamol assay used in this s...

Drosophila melanogaster has recently emerged as a useful model system in which to study the genetic basis of regulation of fat storage. One of the most frequently used methods for evaluating the levels of stored fat (triglycerides) in flies is a coupled colorimetric assay available as a kit from several manufacturers. This is an aqueous-based enzymatic assay that is normally used for measuremen...

Total protein content is a measurement common to many applications in basic science and clinical research. Several different fluorescent techniques are available that eliminate many of the problems associated with the traditional absorbance-based colorimetric methods to measure total protein content. Here we describe the use of fluorescamine to quantitate total protein using the BioTek FL600 fl...

The emergence of multidrug-resistant tuberculosis underscores the need for low-cost, rapid methods to determine the susceptibility of Mycobacterium tuberculosis to antibiotics. A new, rapid, easily read, and inexpensive colorimetric method with a tetrazolium indicator performs this determination as quickly and accurately as the more expensive Alamar Blue technique.

Total protein content is a measurement common to many applications in basic science and clinical research. Several different fluorescent techniques are available that eliminate many of the problems associated with the traditional absorbance-based colorimetric methods to measure total protein content. Here we describe the use of the compound o-phthaldialdehyde (OPA) to quantitate total protein u...

The RGB signals generated by different cameras are not equal for the same scene. Therefore, cameras are characterized based on a CIE standard colorimetric observer. One method of deriving a colorimetric characterization matrix between camera RGB output signals and CIE XYZ tristimulus values is least squares polynomial modeling. Yet, this involves tedious experiments to obtain a camera transfer ...

Spectrophotometric measurement applied on saturated granular activated carbon (GAC) is not yet explored. A colorimetric method in the visible range has been developed in order to determine the exhaustion level of GAC used in rum production. Aqueous ammonia solution has been used as an indicative agent to determine the extraction rate of taste compounds within the rum production process and the ...

An algorithm is proposed for the spectral and colorimetric characterization of digital still cameras (DSC) which allows them to be used as tele-colorimeters with CIE–XYZ color output, in cd/m2. The spectral characterization consists in the calculation of the color-matching functions from the previously measured spectral sensitivities. The colorimetric characterization consists in transforming t...

The present paper studies two different table-based approaches for the calibration of electronic imaging systems. The first approach, which is the classical one,uses the device-independent CIE-XYZ colorimetric space as an intermediate standard space. Input and output devices such as scanners, displays and printers are calibrated separately with respect to the objective CIE-XYZ space. The calibr...