Three commercially available kits that were supplemented with substrates for enzyme reactions were evaluated to determine their abilities to detect coliforms and fecal coliforms in foods. Japanese and U.S. Food and Drug Administration standard methods, as well as two agar plate methods, were compared with the three commercial kits. A total of 50 food samples from various retailers were examined...

background: isolation of high quality genomic dna is one of the most important steps in molecular biology studies related to food borne parasites. usually, various protocols are used for different tissues but so far, there is no common, simple and cost benefit procedure for genomic dna extraction of linguatula serrata ( l. serrata .) larvae as a pentastomida endangering food safety. one of the ...

background :polymerase chain reaction (pcr) assay has widely used for the detection of tuberculosis (tb). this study tried to compare in-house pcr with some well-known commercial pcr kits for detection of tb agent. methods : clinical samples obtained from 620 tb suspected patients were analyzed for the diagnosis of mycobacterium tuberculosis complex (mtc) by in-house pcr. all samples were obtai...