The complex structures of tissues determine their mechanical strength. In engineered tissues formed through self-assembly in a mold, artificially imposed boundary constraints have been found to induce anisotropic clustering of the cells and the extracellular matrix in local regions. To understand how such tissue remodeling at the intermediate length-scale (mesoscale) affects tissue stiffening, ...

Liu, Yu. M.S.M.E., Purdue University, May 2016. Modifications to Johanson’s Roll Compaction Model for Improved Relative Density Predictions. Major Professor: Carl Wassgren, School of Mechanical Engineering. Johanson’s roll compaction model [J.R. Johanson, A rolling theory for granular solids, ASME Journal of Applied Mechanics E32 (1965) 842–848] is modified to improve its predictions of a compa...

The paper presents some results of the characterization of microstuctural differences inside and outside compaction bands in natural sandstone. Specimens of Aztec sandstone were scanned at the synchrotron APS, at Argonne National Laboratory (USA). Porosity measurements obtained by image analysis show a sharp transition inside/outside the compaction band. Finally, preliminary permeability measur...

Interaction between the nascent polypeptide chain and the ribosomal exit tunnel can modulate the rate of translation and induce translational arrest to regulate expression of downstream genes. The ribosomal tunnel also provides a protected environment for initial protein folding events. Here, we present a 2.9 Å cryo-electron microscopy structure of a ribosome stalled during translation of the e...

Soil is a nonrenewable source that needs considerable management to prevent physical deteriorationby erosion and compaction. Compacted soil causes low fertility and yield. The purpose of this study isto investigate the effect of viscoelastic properties of soil and to determine important factors oncompaction. Furthermore, stress distribution, prediction of soil compaction and simulation of its e...

Using small-angle neutron scattering (SANS), we have measured the salt-dependent static structure factor of di- and trinucleosomes from chicken erythrocytes and from COS-7 cells. We also determined the sedimentation coefficients of these dinucleosomes and dinucleosomes reconstituted on a 416-bp DNA containing two nucleosome positioning sequences of the 5S rDNA of Lytechinus variegatus at low an...

Apicomplexans, including the pathogens Plasmodium and Toxoplasma, carry a nonphotosynthetic plastid of secondary endosymbiotic origin called the apicoplast. The P. falciparum apicoplast contains a 35 kb, circular DNA genome with limited coding capacity that lacks genes encoding proteins for DNA organization and replication. We report identification of a nuclear-encoded bacterial histone-like pr...

Mixtures containing ibuprofen (IB) complexed with β-cyclodextrin (βCD) obtained by two complexation methods [suspension/solution (with water removed by air stream, spray- and freeze-drying) and kneading technique] were processed into pharmaceutical dosage forms (minitablets and capsules). Powders (IB, βCD and IBβCD) were characterized for moisture content, densities (true and bulk), angle of re...

Crop rotations that include tap-rooted species of cover crops may help alleviate the deleterious effects of soil compaction on plant growth by modifying soil physical properties. We studied the effects of compaction and cover crops on the least limiting water range (LLWR) and air permeability in the surface layers of a loamy (Exp. 1) and a sandy soil (Exp. 2). There were three compaction treatm...

Organs develop distinctive morphologies to fulfill their unique functions. We used Drosophila embryonic gonads as a model to study how two different cell lineages, primordial germ cells (PGCs) and somatic gonadal precursors (SGPs), combine to form one organ. We developed a membrane GFP marker to image SGP behaviors live. These studies show that a combination of SGP cell shape changes and inward...