Running Title Towards a confocal subcellular atlas of the human proteome Towards a confocal subcellular atlas of the human proteome

We demonstrate quantitative phase mapping in confocal optical microscopy by applying synthetic optical holography (SOH), a recently introduced method for technically simple and fast phase imaging in scanning optical microscopy. SOH is implemented in a confocal microscope by simply adding a linearly moving reference mirror to the microscope setup, which generates a synthetic reference wave analo...

We introduce 4Pi confocal microscopy with destructive interference of converging waves. Linear lobe deconvolution as well as nonlinear restoration of 4Pi confocal raw data with their point-spread functions (PSF's) leads to almost-identical images, irrespective of whether the 4Pi confocal PSF relies on constructive or destructive interference. Three-dimensional imaging of microtubules of a mouse...

A diffractive zone plate provides a highly linear wavelength-to-depth coding, allowing for nonmechanical depth scanning in a confocal microscope. This chromatic confocal microscope, constructed with 40x and 60x objectives, achieves axial position changes of 55 and 25 mum, respectively, for a wavelength tuning range of 100 nm. The corresponding longitudinal point-spread functions are measured an...

Distinguishing between benign and malignant neoplasms of the skin is a daily challenge to dermatologists. With the use of a dermatoscope and other imaging devices, the diagnosis is often more precise. The confocal microscope is a device that uses a near-infrared laser to perform noninvasive imaging of the skin. The benefit is that the images immediately provide additional, cellular-level inform...

Tracheobronchial amyloidosis is one of many causes of endobronchial stenosis and nodularity, the concrete diagnosis of which currently requires the finding of apple-green birefringence from endobronchial biopsies. Bronchoscopic probe-based confocal endomicroscopy (pCLE) is a novel optical biopsy technique which provides real-time images of the lattice structure of the bronchial basement memRece...

The genetically tractable organism Drosophila melanogaster is proving to be an excellent model system for cell biological analysis in the context of the whole organism. The relative ease with which embryos can be obtained in large numbers and processed for highresolution light microscopy has facilitated many recent advances at the interface between cell and developmental biology. Fine subcellul...

Selective-plane illumination microscopy has proven to be a powerful imaging technique due to its unsurpassed acquisition speed and gentle optical sectioning. However, even in the case of multiview imaging techniques that illuminate and image the sample from multiple directions, light scattering inside tissues often severely impairs image contrast. Here we combine multiview light-sheet imaging w...

PURPOSE To compare the relative diagnostic value of confocal microscopy and superficial corneal cultures in the diagnosis of Acanthamoeba keratitis by using clinical and microbiologic definitions of disease. METHODS Results of confocal microscopy, superficial corneal smear, and superficial corneal culture were analyzed for validity against 2 different microbiologic and a clinical composite st...