We determined the acyl and alkyl chain composition of GPI-anchors isolated from MDCK and Fischer rat thyroid (FRT) cells. Both cell lines synthesize GPI-anchors containing C16/C18 or C18/C18 saturated acyl and alkyl chains. The GPI-anchored placental alkaline phosphatase (PLAP) expressed in both cells is raft-associated and PLAP purified from FRT cells is raft-associated in vitro when reconstit...

Large (>1600 microm), ingestively masticated particles of bermuda grass (Cynodon dactylon L. Pers.) leaf and stem labelled with 169Yb and 144Ce respectively were inserted into the rumen digesta raft of heifers grazing bermuda grass. The concentration of markers in digesta sampled from the raft and ventral rumen were monitored at regular intervals over approximately 144 h. The data from the two ...

For realistic virtual dissection, the sectioned images of a cadaver are a desirable material because of their high resolution and real body color. After a volume model is made of the sectioned images, it can be piled or peeled at the intended thickness as if a structure's surface is expanded and shrunken. The purpose of our study was to confirm whether laparoscopic and endoscopic exploration of...

Dielectric elastomer is one of the new types electronic materials that have both drive ability and electric generating capacity. In this research, fundamental tests were performed to evaluate abilities of dielectric elastomer. As the result, it was found that dielectric elastomer had very efficient drive ability and electric generating capacity, and that it was applicable to both slow and high ...

Cell adhesion and spreading require integrins-mediated cell-extracellular matrix interaction. Integrins function through binding to extracellular matrix and subsequent clustering to initiate focal adhesion formation and actin cytoskeleton rearrangement. Lipid raft, a liquid ordered plasma membrane microdomain, has been reported to play major roles in membrane motility by regulating cell surface...

We propose a Byzantine Fault Tolerant variant of the Raft consensus algorithm, BFTRaft, inspired by the original Raft[1] algorithm and the Practical Byzantine Fault Tolerance algorithm[2]. BFT Raft maintains the safety, fault tolerance, and liveness properties of Raft in the presence of Byzantine faults, while also aiming towards to Raft’s goal of simplicity and understandability. We have imple...

Despite the importance of cholesterol in the formation and function of caveolar microdomains in plasma membranes, almost nothing is known regarding the structural properties, cholesterol dynamics or intracellular factors affecting caveolar cholesterol dynamics. A non-detergent method was employed to isolate caveolae/raft domains from purified plasma membranes of murine fibroblasts. A series of ...

The effect on raft foundation of a barrage due to varying stratum has been examined using finite element analysis. The raft foundation is completely resting on soil media and surrounded by soil and rock media. Eight noded brick elements have been used for three-dimensional modeling and meshing of the rock, soil, cut-off, pier, beam and abutment but raft foundation has been meshed using plate el...