this research has focused on isolation and characterization of a strain of bacillus sp. from alkaline soil, which was able to produce extracellular alkaline protease at phs ranging from 8 to 11 and temperatures of 20 to 50ºc. also the impact of different carbon andnitrogen sources were investigated. the yield and fold of enzyme purification was 24% and 50 times, respectively. molecular weight o...

0.094 ----TSB 37°C Baciillus sp. Clone 35 23 0.047 ----TSB 37°C Bacillaceae bacterium C22 21 1.7 ----BA 37°C Bacillus cereus 43 0.75 ----BA 30°C 38 0.5 ----TSB 30°C Bacillus fusiformis 14 1.5 ----BA 37°C Bacillus aquimaris 45 1.2 CXM30 4.25mm BA 30°C Bacillus pumilus 48 3.5 ----R2A 37°C Bacillus megaterium 32 0.064 ----BA 30°C 42 0.75 ----TSB 37°C 19 0.25 ----TSB 30°C 18 0.38 CXM30 1.75mm LB 37°C

1 Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas, Piracicaba, SP, Brazil. 2 Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. Corresponding author: Eliana Dantas da Costa – Avenida Limeira, 901 – Areião – Zip Code: 13414-903 – Piracicaba, SP, Brazil – Phone: (55 19) 2106-5327 – E-mail: [email protected] Received on: Feb 1, 2016 – Accepted on: Ap...

Partially purified α-galactosidase from Bacillus sp. LX-1 was non-covalently immobilized on a reversibly soluble-insoluble polymer, Eudragit L-100, and an immobilization efficiency of 0.93 was obtained. The optimum pH of the free and immobilized enzyme was 6.5 to 7.0 and 7.0, respectively, while there was no change in optimum temperature between the free and immobilized α-galactosidase. The imm...

In this work 2-O-α-D-glucopyranosyl-L-ascorbic acid (AA-2G) was synthesized by Cyclodextrin glucanotransferase (CGTase) from Bacillus sp. SK 13.002 with L-ascorbic acid (AA) as an acceptor and maltose as a glycosyl donor. AA-2G production was analyzed by HPLC and was confirmed by LC/MS results. The reaction parameters, such as pH (4.0-9.0), temperature (25-50°C), time (0-30 h), substrate ratios...

The antimicrobial effects of extracts of A. sativum and P. emblica, against pathogenic Staphylococcus aureus, Escherichia coli, Pseudomonas sp., Klebsiella sp, Salmonella typhi, Salmonella paratyphi, Salmonella sp., Bacillus subtilis, Bacillus cereus were investigated using the agar well and disc diffusion method. Aqueous extracts of A. sativum and P. emblica were more effective than ethanolic ...

A thermostable chitosanase gene from the environmental isolate Bacillus sp. strain CK4, which was identified on the basis of phylogenetic analysis of the 16S rRNA gene sequence and phenotypic analysis, was cloned, and its complete DNA sequence was determined. The thermostable chitosanase gene was composed of an 822-bp open reading frame which encodes a protein of 242 amino acids and a signal pe...