High affinity Ca2+-stimulated Mga+-dependent ATPase activity of nerve ending particles (!vnaphmm) from rat brain tissue appears to be associated primarily with isolated synaptic plasma membranes. The synaptic membrane (Ca2+ + Mga+)-ATPase activity was found to exhibit strict dependence on Mg+ for the presence of the activity, a high affinity for Ca2+ (&.a = 0.23 NM), and relatively high affini...

Vanadium, V, is a redox-sensitive metal that in solution, under aerobic conditions, prevails as the oxyanion vanadate(V). There is little known regarding vanadium toxicity to soil biota, and the present study was set up to determine the toxicity of added vanadate to soil organisms and to investigate the relationship between toxicity and vanadium sorption in soils. Five soils with contrasting pr...

Vanadium biological studies often disregarded the formation of decameric vanadate species known to interact, in vitro, with high-affinity with many proteins such as myosin and sarcoplasmic reticulum calcium pump and also to inhibit these biochemical systems involved in energy transduction. Moreover, very few in vivo animal studies involving vanadium consider the contribution of decavanadate to ...

The detection superoxide production in vascular cells is usually limited by a low sensitivity of available assays. We tested the applicability of the luminol derivate L-012 ¿8-amino-5-chloro-7-phenylpyridol¿3,4-dpyridazine-1,4(2H,3H)dione to measure superoxide production in cultured endothelial cells (human umbilical vein endothelial cells) and rat aortic segments. Following stimulation with t...

Semicarbazide-sensitive amine oxidase (SSAO) is very abundant at the plasma membrane in adipocytes. The combination of SSAO substrates and low concentrations of vanadate markedly stimulates glucose transport and GLUT4 glucose transporter recruitment to the cell surface in rat adipocytes by a mechanism that requires SSAO activity and hydrogen peroxide formation. Substrates of SSAO such as benzyl...

Using the human Intestine 407 cell line as a model, we investigated a possible role for tyrosine kinase(s) in regulating the ion efflux pathways induced by hyposmotic stimulation (regulatory volume decrease, RVD). Pretreatment of 125I(-)-and 86Rb(+)-loaded cells with the phosphotyrosine phosphatase inhibitor sodium orthovanadate (200 microM) potentiated isotope efflux triggered by mild hypotoni...

High affinity Ca2+-stimulated Mga+-dependent ATPase activity of nerve ending particles (!vnaphmm) from rat brain tissue appears to be associated primarily with isolated synaptic plasma membranes. The synaptic membrane (Ca2+ + Mga+)-ATPase activity was found to exhibit strict dependence on Mg+ for the presence of the activity, a high affinity for Ca2+ (&.a = 0.23 NM), and relatively high affini...

The inherent safety and low cost of aqueous aluminum-air (Al-air) batteries have attracted significant attention. However, their lifespan is constrained due to the formation passive layers severe self-corrosion Al anode. This work addresses anode issues using an innovative design strategy by adding vanadate nanoclay modify interaction electrolyte. results shown that each vanadate, nanoclay, a h...