BACKGROUND Cataract surgery after penetratingkeratoplasty (PKP) is often challenging due to changes in the integrity of the cornea caused by PKP. For example, corneal endothelial cell (CEC) loss and corneal edema commonly occur after traditional phacoemulsification cataract surgery in patients that previously had successful PKP. Recent studies have reported that femtosecond laser-assisted catar...

Purpose: To correlate corneal endothelium cell density with dry eye disease compared to an age-matched control group. Materials and Methods: In this cross-sectional study, a total of 150 eyes of 75 female students aged 19-25 years who did not have any history of eye injuries or eye disease affecting the corneal endothelium cell density, were recruited from KSU Female Campus. They were divided i...

Human corneal endothelial cells have a limited ability to replicate in vivo and in vitro. Allograft transplantation becomes necessary when an accident or trauma results in excessive cell loss. The reconstruction of the cornea endothelium using autologous cell sources is a promising alternative option for therapeutic or in vitro drug testing applications. The native corneal endothelium rests on ...

PURPOSE The authors conducted in vivo assessment of corneal endothelial toxicity of air and SF6 in the feline model. This research was motivated by the increased use of air in anterior segment surgery in human subjects. METHODS This was a prospective masked study. The eyes of 16 healthy adult cats were randomly assigned for the injection of 0.7 mL air into the anterior chamber of one eye and ...

BACKGROUND Measurement of corneal endothelial cell density is important both for clinical diagnosis as well as clinical studies. Since endothelial cell loss is considered irreversible in humans, even small changes in endothelial cell density are relevant. Therefore it is important to know whether different instruments for endothelial cell density measurements give the same results and can thus ...

may have been due to the repeated transfers. In later passages, cell growth slowed down (Fig. 2B). The addition of culture grade EGF to the medium resulted in an improved growth rate (Fig. 2D). Gospodarowicz et al observed similar but much greater effects on bovine endothelial cells. The lower response of murine EGF in this study may be due to the species difference. There has been increasing i...

OBJECTIVE To report the outcome of mushroom keratoplasty for the treatment of full thickness corneal disease in pediatric patients with healthy endothelium. METHODS A retrospective analysis of pediatric patients who underwent mushroom keratoplasty. The medical records of pediatric patients suffering from full thickness corneal stromal disease with normal endothelium who underwent mushroom ker...

purpose: to investigate the biomechanical features of transplanted corneas after penetrating keratoplasty (pk), deep anterior lamellar keratoplasty (dalk), and descemet stripping automated endothelial keratoplasty (dsaek) and compare these measurements with those of a normal group. methods: forty-five eyes of 36 patients underwent pk, 23 eyes of 21 patients received dalk using anwar’s big bubbl...

INTRODUCTION Loss of corneal endothelial cells (CECs) is one major factor limiting transplant clarity and survival after keratoplasty. Amongst other factors, apoptosis due to cellular stress is responsible for these problems. This study investigates the possible anti-apoptotic and cytoprotective effects of minocycline on a human corneal endothelial cell line (HCEC-SV40) cultured under oxidative...

Corneal endothelial (CE) cells do not divide in vivo, leading to edema, corneal clouding and vision loss when the density drops below a critical level. The endothelium can be replaced by transplanting allogeneic tissue; however, access to donated tissue is limited worldwide resulting in critical need for new sources of corneal grafts. In vitro expansion of CE cells is a potential solution, but ...