PURPOSE Cystinosis is caused by a deficiency in the lysosomal cystine transporter, cystinosin (CTNS gene), resulting in cystine crystal accumulation in tissues. In eyes, crystals accumulate in the cornea causing photophobia and eventually blindness. Hematopoietic stem progenitor cells (HSPCs) rescue the kidney in a mouse model of cystinosis. We investigated the potential for HSPC transplantatio...

stem cells located at the limbus ,  are the ultimate source for regeneration of the corneal epithelium in the normal and traumatized states.  when limbal stem cells are dysfunctional or deficient, limbal stem cell deficiency (lscd) develops. its surgical management depends on laterality and severity of corneal-limbal involvement. conventional methods of stem cell transplantation are conjunctiva...

purpose: to evaluate the effect of aamniotic mmembrane ttransplantation (amt) in the management of acute severe ocular chemical burns. methods: this randomized clinical trial was performed in eligible patients with grade iii & iv acute chemical burn who randomly received conventional medical therapy (15 eyes) versus combined medical & amniotic membrane transplantation (15 eyes). main outcome me...

purpose: to evaluate the efficacy of riboflavin administration and ultraviolet a (uva) cross-linking on advanced symptomatic bullous keratopathy (bk). methods: 10 patients with symptomatic pseudophakic bullous keratopathy (pbk) were included. slit-lamp examination, visual acuity, foreign body sensation (fbs) and corneal thickness as measured by pentacam, were performed before corneal cross-link...

We report a case of tectonic corneal transplantation for impending corneal perforation to preserve anatomic integrity using cryopreserved donor tissue. An 82-year-old woman exhibiting impending corneal perforation suffered from moderate ocular pain in the left eye for one week. After abnormal tissues around the impending perforation area were carefully peeled away using a Crescent blade and Van...

PURPOSE To evaluate the results of sutureless amniotic membrane (AM) transplantation using fibrin glue for reconstructing corneal surfaces with partial limbal stem cell deficiency (LSCD). DESIGN Retrospective noncomparative interventional case series. METHODS Eleven eyes of nine patients that had LSCD with 120 degrees to almost 360 degrees of limbal involvement underwent superficial keratec...

AIM To examine the efficacy, safety, and long term outcomes of amniotic membrane transplantation for corneal surface reconstruction in cases of partial limbal stem cell deficiency. METHODS 17 eyes of 15 patients with partial limbal stem cell deficiency underwent superficial keratectomy of the conjunctivalised corneal surface followed by amniotic membrane transplantation. Cases were followed u...

With the establishment of human embryonic stem (ES) cell lines, the idea of transplanting stem cells differentiating into blood cells, nerve cells, and muscle cells is gradually being realized. If stem cells established from human ES cells become available, not only transplantation medicine, but also the quality of medicine as a whole would be expected to change. If extracorporeal generation of...

PURPOSE To evaluate the role that delayed-type hypersensitivity (DTH) and cytotoxic T lymphocyte responses play in a murine model of orthotopic corneal allograft transplantation. METHODS Corneal transplantation was performed by grafting C57BL/6 donor corneas into BALB/c corneal beds. After transplantation, the mice were observed by slit lamp biomicroscopy on a weekly basis and graded for sign...