The internal transcribed spacers (ITS) of the nuclear ribosomal DNA and trnL-F region of the chloroplast DNA were sequenced for all ten species of Chloranthus and the outgroup Sarcandra (Chloranthaceae). Parsimony analyses of separate and combined data sets strongly suggest that Chloranthus is monophyletic and can be divided into two major clades: one containing C. erectus, C. spicatus, C. serr...

The genus Crinum L. is the only pantropical genus of the Amaryllidaceae. Phylogenetic and biogeographical analyses of nrDNA ITS and plastid trnL-F sequences for all continental groups of the genus Crinum and related African genera are presented, with the genus Amaryllis used as outgroup. ITS indicates that C. baumii is more closely related to Ammocharis and Cybistetes than to Crinum sensu stric...

An updated subtribal classification of the Compositae–Anthemideae is presented based on gene- and species-tree reconstructions considering sequence variation from plastid (cpDNA ndhF, trnL-trnF) nuclear genome (nrDNA ITS1-5.8S-ITS2, ETS; nDNA dual specificity phosphatase DSP, plus-3 domain-containing protein vernalization independence 5 complex VIP5, major facilitator superfamily Nitrate Transp...

BACKGROUND AND AIMS The phylogenetic relationships between species of Coffea and Psilanthus remain poorly understood, owing to low levels of sequence variation recovered in previous studies, coupled with relatively limited species sampling. In this study, the relationships between Coffea and Psilanthus species are assessed based on substantially increased molecular sequence data and greatly imp...

BACKGROUND Species identification of living organisms by standard DNA sequences has been well-accepted. Consortium for the Barcode of Life (CBOL) recommends chloroplast regions rbcL and matK as the DNA barcodes for the land plants. This study aims to evaluate the feasibility and limitations of rbcL, matK, and 5 other commonly used regions as the DNA barcodes for the medicinal Gentiana and their...

A part of the tRNA(Leu)(UAA) gene containing a 240-nucleotide group I intron was amplified by PCR from cyanobacterium Synechococcus PCC 6301 genomic DNA. The pre-tRNA synthesized from the cloned PCR product was efficiently self-spliced in vitro under physiological conditions. The gene encoding the tRNA(Leu)(UAA), trnL-UAA, was isolated from a Synechococcus PCC 6301 genomic library and the nucle...

Multiple origins of polyploidy from an ancestral diploid plant species were investigated using restriction site polymorphism and sequence variation in the chloroplast DNA (cpDNA) of Heuchera grossulariifolia (Saxifragaceae). Phylogenetic analysis indicated that autopolyploidy has arisen at least twice in the evolutionary history of this species and potentially up to as many as seven times. Thes...

We tested the hypothesis that Uniola paniculata populations are divided into eastern and western lineages, with the primary geographic break at the southern tip of Florida, as observed in codistributed animal taxa. We asked whether the geographic distribution of chloroplast DNA (cpDNA) variation in U. paniculata corresponds to 1) genetic structure in nuclear variation reported in previous studi...

A DNA fiber-based fluorescence in situ hybridization (fiber-FISH) technique was developed to analyze the structure and organization of a large number of intact chloroplast DNA (cpDNA) molecules from Arabidopsis, tobacco, and pea. Using this cytogenomic approach, we determined that 25 to 45% of the cpDNA within developing leaf tissue consists of circular molecules. Both linear and circular DNA f...

In this study, based on molecular biology techniques, the matK and trnL-F regions of ten Annonaceae species grown in Binh Chau-Phuoc Buu Nature Reserve, Vietnam were successfully amplified sequenced for first time. Along with other highly homologous DNA sequences from GenBank database, phylogeny studied was also established. By using alignment tool NCBI percentage identity among presented. The ...