The measurement of creatinine in serum is a key indicator of glomerular function of the kidney and its pivotal role in this evaluation has been further increased by recommendations issued by nephrology societies for using equations to estimate the glomerular filtration rate (GFR) [1]. In 2006 the reference system for the measurement of serum creatinine, using suitable higher-order reference mat...

We found that urinary fibronectin (UFN) in cancer patients was almost all fragmented and consisted mainly of the cell-binding domain. We developed a two-site immunoenzymometric assay for UFN, using two monoclonal antibodies that both recognize this domain of fibronectin. The amount of UFN was expressed as arbitrary units per milligram of creatinine. Some 2% of the 623 healthy subjects had UFN a...

We describe a "high-performance" liquid-chromatographic method for determining 3-methoxy-4-hydroxyphenylethylene glycol (MHPG) in human urine. MHPG is separated on a reversed-phase column with isocratic elution, oxidized with sodium metaperiodate, and its absorbance measured at 365 nm. This method shows higher specificity, less interference for MHPG than methods involving electrochemical or flu...

Aim—To investigate factors that influence urinary tissue factor (uTF) measurements: glomerular permeability and filtration, tubular function, haematuria, and urine bacterial growth. Methods—uTF, protein creatinine index, glomerular filtration rate, retinol binding protein, N-acetyl-â-D-glucosaminidase (NAG) and urinary haemoglobin (uHb) were measured in patients with hypertension, diabetes mell...

Spreadsheets may be created to include the Cockcroft-Gault Formula (CGF) for creatinine-based estimation of glomerular filtration rate. Creatinine clearance (CrCl) provides a more accurate method for perioperative risk assessment of renal function than serum creatinine. CrCl may be used to develop guidelines for renal protective management strategies during cardiopulmonary bypass. CGF uses seru...

Thirteen newborn infants, 8 preterm and 5 term, with either mild or serious neonatal asphyxia were studied. From the first 24 hours of life to day 13, glomerular filtration rate (GFR) estimated by creatinine clearance was compared with the values obtained using Schwartz's formula: GFR (ml/min per 1.73 m2) = 0.55 x length (cm)/plasma creatinine (mg/100 ml). Both in term and preterm infants, valu...

AIM To investigate factors that influence urinary tissue factor (uTF) measurements: glomerular permeability and filtration, tubular function, haematuria, and urine bacterial growth. METHODS uTF, protein creatinine index, glomerular filtration rate, retinol binding protein, N-acetyl-beta-D-glucosaminidase (NAG) and urinary haemoglobin (uHb) were measured in patients with hypertension, diabetes...

Aim of this study is to confirm and investigate the diuretic effect of crocine which is the pharmacologically active component of Crocus sativus L. (saffron) and to study the possible mechanism of action in relation to urinary nitrite. Twenty four rats were divided into 3 groups; control, hydrochlorothiazide and crocine treated groups. Assay of the diuretic activity of crocine was performed by ...

Aim of this study is to confirm and investigate the diuretic effect of crocine which is the pharmacologically active component of Crocus sativus L. (saffron) and to study the possible mechanism of action in relation to urinary nitrite. Twenty four rats were divided into 3 groups; control, hydrochlorothiazide and crocine treated groups. Assay of the diuretic activity of crocine was performed by ...

Methods Objective: Th e fi rst objective of the present study was to determine whether an NGAL immunoassay developed for a standardized clinical platform (ARCHITECT® analy zer, Abbott Diagnostics) correlates with the researchbased assay. Th e second objective was to determine the utility of the standardized NGAL immunoassay as a predictive biomarker of AKI after CPB in a large prospective cohor...