Background/Aims: The use of novel cryo-additive agents to increase cell viability post-cryopreservation is paramount improve future based-therapy treatments. We aimed establish the Human Leukemia (HL-60) cells lipidomic and biological patterns when cryo-preserved in DMSO alone with 300 µM Nigerose (Nig), 200 Salidroside (Sal) or a combination Nig (150 µM) Sal (100 µM). Methods: HL-60 were pre-i...

Background: More than a decade work in preservation of male fertility by spermatogonial stem cell (SSC) transplantation in animal models as well as new discoveries about the nature of human SSCs has prompted an investigation into the possibility of an effective clinicalgrade procedure for isolation and cryopreservation of human testicular cells. Materials and Methods: Clinical-grade reagents, v...

two experiments were designed to examine the effects of crude seminal plasma (csp) exposure ofejaculated and epididymal spermatozoa before freezing. experiment 1, two consecutive ejaculates werecollected (n=4) within 5 min, the second one was carried out in a tube containing fiser extender (coatedspermatozoa); by centrifugation, seminal plasma was removed from coated ejaculates. the pellets wer...

PURPOSE The previous in vitro study showed that trehalose, when used as a cryoprotective agent (CPA) in an optimal concentration, can provide adequate protection of adipose aspirates during cryopreservation. OBJECTIVE The authors evaluated the efficacy of trehalose in its optimal concentration for cryopreservation of human fat grafts in a well-established animal model. METHODS In this study...

Oil palm (Elaeis guineensis) is the highest oil-yielding commercially grown perennial tree. germplasm conservation and in vitro clonal propagation strengthened world’s efforts to ensure future food security. Cryopreservation provides long-term storage for germplasm. The of plant material at cryogenic temperatures (−196 °C) following dehydration causes cryoinjury. cryotolerance mechanism has rar...

STUDY QUESTION Is there a better alternative to the conventional cryopreservation protocols for human testicular tissue banking? SUMMARY ANSWER Uncontrolled slow freezing (USF) using 1.5 M dimethylsulphoxide (DMSO) and 0.15 M sucrose as cryoprotectants appears to be a user-friendly and efficient method for the cryopreservation of human testicular tissue. WHAT IS KNOWN ALREADY Currently, tim...

Spermatozoa cryopreservation is an effective method for maintaining male fertility in humans. Nevertheless, there are some limitations of sperm cryopreservation, which called as cell injury by cryoprotectant, that cannot be avoided. This process will affect embryo quality. Therefore, it mandatory to modify cryoprotectant spermatozoa improve review aimed summarize the modification can damage cel...

The present study evaluates the effect that various steps of a conventional cycle of cryopreservation have on the patterns of movement exhibited by boar spermatozoa. Sperm-rich ejaculate fractions collected from 24 mature fertile boars (1 ejaculate per boar) were cryopreserved following a standard freeze-thaw procedure with 0.5-mL plastic straws. Overall sperm motility and the individual kinema...