The number of reported new cancer cases is increasing every year. The probability of surviving cancer is high and is continually improving. The cancer treatment may induce ovarian or testicular failure by damaging ovarian follicles in females and spermatogonia in the males. Gonadal failure may affect all aspects of reproductive health, including pubertal development, hormone production, and sex...

BACKGROUND Intracytoplasmic sperm injection (ICSI) allows pregnancies to be established with a single sperm, improving the chances for men with severely impaired sperm quality to cause a pregnancy. Men with leukemia typically are of reproductive age and their fertility is threatened by initially impaired semen quality and cytotoxic chemotherapy. The authors examined the feasibility of sperm cry...

Introduction: Infertility is a common phenomenon in modern societies. Today, use of assisted reproductive technologies (ARTs) for treatment of infertility is common, and cryopreservation is one of these technologies. Cryopreservation has impact on the function and percentage of fertility of human sperm. In this study, motility, viability, nitric oxide, and DNA apoptosis were assessed before and...

Cryopreservation is required to preserve the native properties of tissue for prolonged periods time. In this study, we evaluate impact that 4 different cryopreservation protocols have on porcine urethral tissue, identify a protocol best preserves tissue. The include storage in cryoprotective agents at ?20 °C and ?80 with slow, gradual, fast reduction temperature. To effects cryopreservation, me...

Cryopreservation of sperm has many applications in agriculture, biotechnology, and clinical medicine. The spermatozoon is a very specialized cell that loses the ability of biosynthesis, repair, growth and cell division during the final phase of spermatogenesis. Cryopreservation of sperm generally requires a reduction or arrest of the metabolism of cells, thereby prolonging their life. Semen sam...

Cryopreservation has the capacity to extend spermatozoa's lifespan and viability. In addition, the semen samples can be collected, preserved and stored or sent to distant locations and still be used long after the death of the semen donor. In this study for the vitrification of dog sperm (fresh and swum-up sperm), different cryopreservation mediums on the basis of glycerol, milk and egg yolk we...

The first clinical trials with adoptive Treg therapy have shown safety and potential efficacy. Feasibility of such therapy could be improved if cells are cryopreserved and stored until optimal timing for infusion. Herein, we report the evaluation of two cell-banking strategies for Treg therapy: 1) cryopreservation of CD4+ cells for subsequent Treg isolation/expansion and 2) cryopreservation of ...

A novel and simple procedure for the controlled-rate cryopreservation of peripheral blood progenitor cells (PBPCs) was introduced. A freezing bag housed in a protective aluminum canister was placed on top of a styrene foam box in the -85 degrees C electric freezer. A second set of samples was kept in cryotubes placed in a double styrene foam box in the same electric freezer. Measurement of the ...

Ex situ conservation is critical for hedging against the loss of plant diversity. For those species (exceptional species) that cannot be conserved long-term in standard seed banks, alternative methods are required, often involving in vitro culture and cryopreservation, or storage in liquid nitrogen. Cycladenia humilis var. jonesii is a federally threatened perennial native to Utah and Arizona. ...

BACKGROUND Cryopreservation of semen requires optimized conditions to minimize the harmful effects of various stresses. The main approach for protection of sperm against stress is based on the use of antioxidants and cryoprotectants, which are described as defensive methods. Recently, the application of controlled mild stressors has been de- scribed for activation of a temporary response in ooc...