Development of protocols for cryopreservation microalgae Chlorococcum dissectum Korshikov and Dunaliella salina Teodoresco requires selection effective cryoprotectants their concentrations. One the stages choosing optimal concentrations is determining degree toxicity cells at equilibration stage. Solutions dimethyl sulfoxide, ethylene glycol, ethanol, glycerol (5–30%) modified PVS1 PVS2 (50 75%...

This study aimed to establish a cryopreservation protocol for embryogenic cultures of A. angustifolia, enabling the ex situ conservation of the species. Embryogenic cultures were established from immature seeds and treated with variations of the cryoprotectant solutions SuDG, SoD and PVS2 prior to immersion in liquid nitrogen. Cell viability was evaluated after 30, 60 and 90 days of re-growth. ...

The Southampton In Vitro Fertilisation Programme was established in 1986. Since that time the programme has maintained a clinical pregnancy rate of over 25 per oocyte retrieval. Embryos in excess of requirements for fresh transfer have been cryopreserved using controlled rate freezing and Propanediol as a cryoprotectant. Review of the first 75 frozen embryo transfers in natural or clomid induce...

Cryoprotectants have critical roles to prevent cell damages during cryopreservation. However, the adjustment of cryoprotectant concentration is also very crucial protect cells from toxicity. The present study was designed investigate effect extenders including high dimethyl sulfoxide (DMSO) on post-thaw rabbit sperm quality. Pooled samples (n=7) were diluted and cryopreserved in 250 mmol/L Tris...