BACKGROUND Thymoquinone is an active principle of Nigella sativa seed known as "Habbah Al-Sauda" in Arabic countries and "Sinouj" in Tunisia. Bacterial biofilms tend to exhibit significant tolerance to antimicrobials drugs during infections. METHODS The antibacterial activity of Thymoquinone (TQ) and its biofilm inhibition potencies were investigated on 11 human pathogenic bacteria. The growt...

Objective The objective of the study is to investigate biofilm forming ability, distribution of biofilm associated genes of the clinically isolated bovine mastitis Staphylococcus and the correlation of them. Methods Silver staining, SEM and crystal violet staining were conducted for the detection of biofilm forming ability in 24-well plates. Genes of bap, icaAD, icaBC, sar, agr, sigB, clfaA, cl...

Bartholomew, J. W. (University of Southern California, Los Angeles), Thomas Cromwell, and Richard Gan. Analysis of the mechanism of Gram differentiation by use of a filter-paper chromatographic technique. J. Bacteriol. 90:766-777. 1965.-Data are presented which demonstrate that the mechanism of gram-positivity could not be due solely to factors such as a single, specific gram-positive substrate...

The migration of three human prostate tumor epithelial cell lines (TSU-pr1, PC-3, DU-145) in response to secreted protein from a human prostate stromal cell line was investigated by using the modified blind-well Boyden chamber assay. Migrated cells were quantified by spectrophotometrically measuring the concentration of crystal violet stain extracted from their nuclei. Cell number was correlate...

In this study we evaluated the crystal violet decolorization assay (CVDA) for detection of minimum inhibitory concentration (MIC) of antituberculosis drugs. 53 isolates were tested in this study and 13 of them were multidrug resistant (MDR) isolates. The antibiotics concentrations were 2-0.06 mg/L for isoniazid (INH) and rifampicin (RIF) and were 16-0.25 mg/L for streptomycin (STM) and ethambut...

The results 1 from measurements made with the glass electrode and spectrophotometer show that brilliant cresyl blue penetrates as free base into the vacuoles of living cells of Nitella. The present paper describes similar experiments with azure B, methylene blue, and crystal violet. Since the methods for the condition of cells, extraction of the sap, penetration experiments, extraction of free ...

AIMS  To develop a novel Vero cell assay that implements a real-time cell electronic sensing (RT-CES) system for the determination of the presence of verotoxin-producing Escherichia coli (VTEC). The assay overcomes the major drawbacks in conventional Vero cell assay, for example, labour-intensive and time-consuming. METHODS AND RESULTS  Cells were grown onto the surfaces of microelectronic se...

It has been shown previously (Kennedy and Barbaro, 1953) that the amount of crystal violet adsorbed by bacteria can be measured quantitatively by either one of two procedures. One procedure involved micro-Kjeldahl analyses of bacterial cells before and after staining. The increase in total nitrogen in the stained cells represented the dye nitrogen adsorbed. The second procedure was based on mic...

Both the presence and impact of biofilms have proved to be ground breaking regarding early diagnosis and treatment in acute and chronic cutaneous, neurological and other internal diseases. Further, biofilms and the activation of the innate immune system have added clarity to the pathogenesis of those diseases. Our current observations are the first to demonstrate viral biofilms in skin disease;...

PURPOSE The aim of this in vitro study was to investigate the adhesion of initial colonizer, Streptococcus sanguis, on resin, titanium and zirconia under the same surface polishing condition. MATERIALS AND METHODS Specimens were prepared from Z-250, cp-Ti and 3Y-TZP and polished with 1 µm diamond paste. After coating with saliva, each specimen was incubated with Streptococcus sanguis. Scannin...