BACKGROUND Oligoclonal bands (OCB) are the most widely used CSF test to support the diagnosis of MS and to predict conversion of clinically isolated syndrome (CIS) to multiple sclerosis (MS). Since OCB tests are based on non-quantitative and difficult to standardise techniques, measurement of immunoglobulin kappa free light chains (KFLC) may represent an easier to use quantitative test. METHO...

AIM To report on the cerebrospinal fluid (CSF) exchange between the intracranial spaces (ie, basal cisterns) and the subarachnoid space (SAS) of the optic nerve (ON) in subjects with normal-tension glaucoma (NTG) compared with control subjects without NTG or other forms of glaucoma. METHODS CT cisternography of the brain and orbits was performed in 18 patients with NTG (7 women, 11 men; mean ...

Introduction: Tubercular meningitis (TBM) remains a major global health problem and is the most dangerous form of extra-pulmonary tuberculosis. Therefore early, simple and inexpensive diagnostic test has always been a matter of concern especially in a developing country like India. Therefore we aimed in our study to find out the diagnostic significance of cerebrospinal fluid (CSF) and blood ade...

Methods An ongoing prospective study conducted from June 2012October 2013 in 90 HIV seropositive patients presenting with various complaints. CSF and lymphnode aspirates were subjected to preliminary microscopic examination, c (Giemsa staining, mucicarmine staining, negative staining and Gram’s staining). Latex agglutination test was done for CSF samples. Sampls inoculated on SDA at 37C and 25C...

The production of colony-stimulating factors (CSF) is delicately controlled through a complex network of humoral and environmental factors. We have studied some of the mechanisms which regulate the production of CSF as compared to general protein synthesis in the lung tissue ill vitro. When lung tissue from mice was cultured for various times in serum free medium, the first detectable le...

Patients with idiopathic normal pressure hydrocephalus (INPH) have a disturbance in the cerebrospinal fluid (CSF) system. The treatment is neurosurgical – a shunt is placed in the CSF system. The infusion test is used to assess CSF system dynamics and to aid in the selection of patients that will benefit from shunt surgery. The infusion test can be divided into three parts: a mathematical model...

BACKGROUND Herpes simplex encephalitis (HSE) often leads to severe disability or death. Factors usually associated with outcome include Simplified Acute Physiology Score, age and delay of initiation of acyclovir treatment.Our aim was to determine the impact of Herpes simplex virus (HSV) load in cerebrospinal fluid (CSF) upon HSE outcome. METHODS We retrospectively determined HSV load in the C...

650 October 2014, Vol. 104, No. 10 Use of the Xpert MTB/RIF assay in the diagnosis of tuberculous meningitis: A cautionary note To the Editor: The Xpert MTB/RIF (Cepheid) assay is a nucleic acid amplification test developed to detect mycobacterial tuberc­ ulosis (MTB) infection and rifampicin resistance. It is a closed system, requires minimal training to use, and produces a result in a few hou...

Meningitis is an inflammation of the membranes that surround the brain and spinal cord, thereby involving the arachnoid, the pia and the cerebrospinal fluid (CSF). It is divided into viral and bacterial meningitis. For different reasons the diagnosis of bacterial meningitis is very important. The examination of CSF samples may provide information about causative microorganism. The sensivity of ...

Background The ratio Aβ42/Aβ40 in cerebrospinal fluid (CSF) is considered one of the most reliable biomarkers Alzheimer’s Disease. ABtest40-IA and ABtest42-IA have been widely used for amyloid-β (Aβ) quantification plasma samples. aim this study was to validate these tests Aβ40 Aβ42 CSF. Method Analytical validation accomplished following FDA EMA reference guidelines. Calibration curves, sensit...