Conventional propidium iodide (PI) staining requires the execution of multiple steps prior to analysis, potentially affecting assay results as well as cell vitality. In this study, this multistep analysis method has been transformed into a single-step, non-toxic, real-time method via live-cell imaging during perfusion with 0.1 μM PI inside a microfluidic cultivation device. Dynamic PI staining ...

BACKGROUND Ginseng (the roots of Panax ginseng Meyer) is a well-known traditional Oriental medicine and is now widely used as a health food. It contains several types of ginsenosides, which are considered the major active medicinal components of ginseng. It has recently been reported that the qualitative and quantitative properties of ginsenosides found in ginseng may differ, depending on culti...

Hydrogenotrophic methanogens are an intriguing group of microorganisms from the domain Archaea. Methanogens exhibit extraordinary ecological, biochemical, and physiological characteristics and possess a huge biotechnological potential. Yet, the only possibility to assess the methane (CH4) production potential of hydrogenotrophic methanogens is to apply gas chromatographic quantification of CH4....

The large scale in vitro cultivation method of Fairlamb et al (1985) was modified to contain human plasma-supplemented medium in HEPES buffer. After 4 days with no change of medium nor agitation of the culture flask, 27- to 50-fold increase in the starting parasitemia of trophozoites were obtained.

The determination of niacin in orange juice with lyophilized Lactobacillus arabinosus ATCC 8014 has the advantage over the usual method in eliminating the delay caused by the necessity of cultivating cells of L. arabinosus for the inoculum and also in eliminating the periodical cultivation on agar slopes.

Crop cultivation in controlled environment plant factories offers great potential to stabilize the yield and quality of agricultural products. However, many crops are currently unsuited to these environments, particularly closed cultivation systems, due to space limitations, low light intensity, high implementation costs, and high energy requirements. A major barrier to closed system cultivatio...

Increased droughts in southern Africa have noticed some appreciation of the role that partial wetland cultivation can play to address household food security. This has also witnessed some indication of possible relaxation of wetland cultivation restrictive policies in Zimbabwe. However, the general perceptions of society towards wetland cultivation remain unclear and critically important for po...

Grazing and cultivation are two important management practices worldwide that can cause significant soil organic carbon (SOC) losses. However, it remains elusive how soil microbes have responded to soil carbon changes under these two practices. Based on a four-decade long field experiment, this study investigated the effects of grazing and cultivation on SOC stocks and microbial properties in t...

The present article is aimed at study on agroclimatic conditions of cultivation of sunflower throughout selected substations at Kurdistan  Province by means of GIS system. In this investigation, meteorological data have been received from synoptic stations based on daily, monthly, and annually trend from Iran Meteorological Organization (IMO) at Kurdistan  Province and then homogeneity of data ...