The sandwich culture of hepatocytes, between double layers of extra-cellular matrix (ECM), is a well-established in vitro model for re-establishing hepatic polarity and maintaining differentiated functions. Applications of the ECM-based sandwich culture are limited by the mass transfer barriers induced by the top gelled ECM layer, complex molecular composition of ECM with batch-to-batch variati...

Hands play a critical role in the transmission of microbiota on one's own body, between individuals, and on environmental surfaces. Effectively measuring the composition of the hand microbiome is important to hand hygiene science, which has implications for human health. Hand hygiene products are evaluated using standard culture-based methods, but standard test methods for culture-independent m...

Molecular information about the bacterial composition of a coculture capable of sulfate reduction after exposure to oxic and microoxic conditions was used to identify and subsequently to isolate the components of the mixture in pure culture. PCR amplification of 16S ribosomal DNA fragments from the coculture, analyzed by denaturing gradient gel electrophoresis, resulted in two distinct 16S ribo...

BACKGROUND Microalgae have been recognized as a good food source of natural biologically active ingredients. Among them, the green microalga Euglena is a very promising food and nutritional supplements, providing high value-added poly-unsaturated fatty acids, paramylon and proteins. Different culture conditions could affect the chemical composition and food quality of microalgal cells. However,...

We analyzed DNA damage, mitotic activity and polyploidization in Crepis capillaris callus cells during shortand long-term in vitro culture, and the influence of plant growth regulators on these processes. Changes in the concentration of growth regulators altered the stability of callus. The level of DNA damage was highly dependent on the growth regulator composition of the medium. Cytokinin at ...

Growth parameters and biochemical composition of the green microalga Chlorella vulgaris cultivated under different mixotrophic conditions were determined and compared to those obtained from a photoautotrophic control culture. Mixotrophic microalgae showed higher specific growth rate, final biomass concentration and productivities of lipids, starch and proteins than microalgae cultivated under p...

persian shallot, also called leek (allium ampeloprasum), is a monocotyledon plant of the lily family (liliaceae). it belongs to the genus allium, has a characteristic taste and morphological features, and is considered as one of the oniony vegetables. this research was conducted with the purpose of obtaining optimal conditions for tissue culture of persian shallot and of comparing calli and lea...

Little is known about the growth performance of sex-reversed, all male Oreochromis niloticus under different traditional and non-traditional culture methods practiced in India. In this study, 17"methyltestosterone treated monosex tilapia was cultured in concrete tank, flow-through system, cage, pen and earthen pond. Similar feeding regime and stocking density of fish were maintained for all the...