OBJECTIVE To compare sensitivity of inner cell mass (ICM) outgrowth assay and analysis of culture media amino acid turnover with the sensitivity of the human sperm motility assay (HSMA) and murine embryo assay (MEA) for detection of formaldehyde toxicity. DESIGN Prospective in vitro study. SETTING University hospital-based infertility center. ANIMAL(S) Murine embryos. INTERVENTION(S) Th...

Phage-peptides were prepared from L. lactis ssp. lactis ml3 and L. plantarum yit0068 bacteriophage using a ficin hydrolysis. The ml3 and yit0068 peptides were compared to L. lactis ssp. lactis c2 bacteriophage peptides to determine growth inhibition of C2 host and inhibition of both ml3 and c2 bacteriophage. M17 with .011 mM CaCl2 media with and without phage peptides (2 or 2.5%) were heat trea...

Molecular methods are increasingly used to identify microbes in clinical samples. A common technical problem with PCR is failed amplification due to the presence of PCR inhibitors. Initial attempts at amplification of the bacterial 16S rRNA gene from inoculated blood culture media failed for this reason. The inhibitor persisted, despite numerous attempts to purify the DNA, and was identified as...

This article reports a replication and extension of a study that explored individual perceptions of factors that underlie the use of electronic media (electronic mail, world-wide-web, list serves, and other collaborative systems). The original study was conducted in a single Australian university. The study was replicated in a Canadian university. The replication allowed testing of the enlarged...

This comparative study of young adults in an established democracy (America) and transitional democracy (Hong Kong) analyzed the impact of political efficacy on the relationship between Internet/Facebook use on political and civic participation. Regression analyses in both samples showed that Facebook use consistently predicted both types of participation. Moreover, there were significant negat...

In an effort to axenically culture the previously uncultivable populations of the rhizobacteria of Lucerne (Medicago sativa L.), we propose plant-only teabags culture media to mimic the nutritional matrix available in the rhizosphere. Here, we show that culture media prepared from Lucerne powder teabags substantially increased the cultivability of Lucerne rhizobacteria compared with a standard ...

AIMS To assess the best medium for primary isolation of enteric pathogens; to determine the need for a second primary culture medium; and to gather information on stool culture media used in 20 other randomly selected laboratories. METHODS Specimens were cultured on desoxycholate citrate agar (DCA), Hektoen enteric agar (Hektoen), and xylose lysine deoxycholate agar (XLD). Non-lactose ferment...

introduction: umbilical cord blood (cb) has been identified as a rich source for hematopoietic stem cells (hscs), and has provided an alternative to bone marrow transplantation. the use of ex vivo expanded cells has been suggested as a possible means to accelerate the speed of engraftment in cord blood (cb) transplantation. the main aim of our study is to find the best culture media and conditi...

The objective of the present study was to investigate the effects of three different culture media on the development of canine somatic cell nuclear transfer (SCNT) embryos. Canine cloned embryos were cultured in modified synthetic oviductal fluid (mSOF), porcine zygote medium-3 (PZM-3), or G1/G2 sequential media. Our results showed that the G1/G2 media yielded significantly higher morula and b...