Development of serum-free suspension cell culture processes is very important for influenza vaccine production. Previously, we developed a MDCK suspension cell line in a serum-free medium. In the present study, the growth kinetics of suspension MDCK cells and influenza virus production in the serum-free medium were investigated, in comparison with those of adherent MDCK cells in both serum-cont...

OBJECTIVES The aim of the present study was to examine the protective action of the antioxidant dimethyl sulfoxide (DMSO) against the oxidative stress on keratinocyte cultures caused by glucose deprivation and hypoxia, using the concentration of malonyl dialdehyde existing in the cell culture as an indicator of the oxidative stress level. METHODS EIGHTY FLASKS WITH CULTURED HUMAN KERATINOCYTE...

Embryogenic cultures of Araucaria angustifolia were established in a BM liquid medium supplemented with 2 μM 2,4-dichlorophenoxyacetic acid, 1 μM 6-benzylaminopurine and 1 μM kinetin (BM2) and in a BM medium free of growth regulators (BM0). During 42 days in culture, the cell growth pattern of both cultures was similar. The pH of the culture medium of both BM0 and BM2 underwent progressive redu...

This study involves in vitro androgenesis of Zea mays L. via anther culture. In the first experiment, three embryo induction media were tested in ETH-M82 genotype of maize. The results showed that the embryo structures were established 2 to 3 weeks after culturing the anthers. The effect of induction medium was signifi­cant for embryo frequencies. The obtained results confirm that the induction...

the use of in vitro potato shoot cultures for protoplast isolation is desirable for consistent protoplast qualities. ethylene build-up in the culture vessels causes problems for leaf growth (e.g. decreasing leaf surface, dry weight and chlorophyll) in shoot culture. fifty to one hundred µm silver thiosulfate (sts) produced a larger leaf area as a source of plant material for protoplast isolatio...

The use of 10% oxgall and bile broth medium, both supplemented with freshly prepared 100 u/ml streptokinase, for isolating Salmonella typhi by clot culture technique was evaluated and compared against whole blood culture systems (3 ml blood in 9 ml media and 8 ml blood in 72 ml media). These gave a 1:4 and 1:10 blood to medium ratio, respectively. Clot cultures in 10% oxgall (CLOX) gave a 57% p...

Mangueira Lagoon, located in the extreme south of Brazil, has water with physicochemical characteristics such as alkaline pH and carbonate levels propitious for the growth of the cyanobacterium Spirulina platensis. Previously published studies have shown that Mangueira Lagoon water supplemented with small quantities of carbon and nitrogen is suitable for S. platensis cultivation and can signifi...

OBJECTIVE To compare three different in vitro maturation (IVM) media for immature oocytes. DESIGN Experimental study. SETTING In vitro fertilization laboratory. ANIMAL(S) BDF1 female and male mice. INTERVENTION(S) Retrieval and maturation of cumulus-enclosed germinal vesicle-stage oocytes according to one of three protocols: group A, conventional IVM medium; group B, blastocyst culture ...

background: this study was designed to evaluate whether or not t. gondii and its derivatives can change the gene expression level of il-10 in murine leukocytes in vivo.   methods: fifty balb/c mice were divided into 5 groups, four of which received the excretory/secretory product (esp) from cell culture medium, the esp from cell free medium, the toxoplasma lysate product (tlp) and the active ta...

Most of the systematic studies used in the development of human embryo culture media have been done first on mouse embryos. The general use of NMRI outbred mice is a model for toxicology, teratology and pharmacology. NMRI mouse embryo exhibit the two-cell block in vitro. The objective of this study was to evaluate and compare the effects of four kinds of culture media on the development of zygo...