BACKGROUND The aim of this study was to assess effects of cysteamine as an anti-oxidant on rate of in vitro maturation of oocyte and determination of its effects on spindle size and shape. METHODS Pre-mature mice were primed with pregnant mare stimulating gonadotrophin (PMSG) and germinal vesicle (GV) stage oocytes were obtained 48 h after. The oocytes were cultured in tissue culture medium (...

Adenyl Cyclase in Human Leukocytes: Evidence for Activation by Separate Beta Adre nergic and Proataglandin Receptors. HENRY R. BOURNEANDKENNETH L. MEL MON 1 The Fate of Intracisternally Administered Norepinepb.rine-1H in the Brain and Spinal Cord of the Rabbit. J. P. CHALMERS AND R. J. WURTMAN .... 8 Studies on the Relationship between 5-Hydroxytryptamine Turnover in Brain and Tolerance and Phy...

BACKGROUND Immunogold cytochemistry is the method of choice for precise localization of antigens on a subcellular scale. The process of immunogold quantification in electron micrographs is laborious, especially for proteins with a dense distribution pattern. NEW METHODS Here I present a MATLAB based toolbox that is optimized for a typical immunogold analysis workflow. It combines automatic de...

The aim of this study was to determine the extent to which sulfate incorporated into biosynthesized basement membrane (BM) components increased as isolated type II cells progress toward a more type I cell-like phenotype from 7 to 21 days in culture. Specific sulfate cytochemistry, using high iron diamine, showed that type I-like cells in 21-day cultures deposited a more highly sulfated extracel...