Background: Sperm mediated gene transfer was approved for producing transgenic animals. There are many studies which reported different success rate by this procedure. Failure in this project mostly is returned to transfection and stability of transferred genes. The other problem that may contribute in project failure may return to the testicular damages which occur at the time of transfection,...

Intracytoplasmic sperm injection (ICSI) is a delicate procedure requiring considerable skills of the person performing it. Theoretically, the injection procedure could damage cytoplasmic structures in the oocyte, resulting in sublethal cellular injury and/or numerical chromosomal abnormalities that could lead to impaired embryonic development. In the present study, features of the injection pro...

PURPOSE We evaluated the retrieval rates and reproductive outcomes of percutaneous sperm retrieval according to the cause of obstructive azoospermia. MATERIALS AND METHODS We retrospectively studied the records of 146 men with obstructive azoospermia who underwent sperm retrieval for intracytoplasmic sperm injection. Patients were grouped by the cause of obstruction, including 32 with congeni...

Received on: April 28, 2009 – Accepted on: Oct 15, 2009 aBstRact Azoospermia is defined as the absence of mature spermatozoa in semen analysis after centrifugation. The causes of azoospermia may be related to spermatogenesis defects and classified as nonobstructive azoospermia, or to the obstruction of the efferent ducts of the genital tract and classified as obstructive azoospermia. Before the...

Injection of eggs of various species with an extract of sperm cytoplasm stimulates intracellular Ca(2+) release that is spatially and temporally like that occurring at fertilization, suggesting that Ca(2+) release at fertilization may be initiated by a soluble factor from the sperm. Here we investigate whether the signalling pathway that leads to Ca(2+) release in response to sperm extract inje...

BACKGROUND Intracytoplasmic sperm injection (ICSI) is an advanced technique employed in assisted reproductive clinics for treatment of infertile couples. The reproductive endocrinologists try their level best to identify factors that enhance success rate after ICSI. OBJECTIVE To compare estradiol progesterone ratio on ovulation induction day amongst pregnancy outcome groups following ICSI. ...

OBJECTIVE To observe effect of peak estradiol (E2) levels on outcome after intra cytoplasmic sperm injection (ICSI). METHODS Quasi experimental design conducted in "Islamabad Clinic Serving Infertile Couples" from June 2010 till August 2011. Down regulation with mid luteal suppression of 564 females aged 18-41 years was done with gonadotrophin releasing hormone agonist followed by controlled ...

Conflicting data still exist regarding the extent of paternal pronuclear DNA demethylation in one cell-stage mammalian embryos. Demethylation of paternal pronuclear DNA was observed in in vivo produced porcine zygotes, whereas in vitro produced embryos do not show any or only weak paternal genome demethylation. In our experiments, we have used and compared two in vitro techniques commonly used ...

Once deposited in the female tract, sperm face a series of challenges that must be overcome to ensure the presence of an adequate normal sperm population close to the site of fertilization. Our aim was to evaluate the influence of the uterine milieu on boar sperm morphology. In experiment 1, sperm morphology was evaluated in the backflow (60 min after insemination) and within the uterotubal jun...

Bulls treated with ethylene dibromide (EDB), either by oral administration of ten doses of 4 mg/kg body weight on alternate days or by a single intraperitoneal injection of 110 -2 mg of this compound into the fluid surrounding each testis, exhibited sperm malformations up to total disintegration of the sperm cells. The malformations appeared in the ejaculates collected about two weeks after the...